RECOMBINANT HUMAN LEUKEMIA INHIBITORY FACTOR IS MITOGENIC FOR HUMAN BONE-DERIVED OSTEOBLAST-LIKE CELLS

被引：14

作者：

EVANS, DB ^{[1
]}

GERBER, B ^{[1
]}

FEYEN, JHM ^{[1
]}

机构：

[1] UNIV BASEL,FELIX PLATTER SPITAL,ORTHOPAED KLIN,BASEL,SWITZERLAND

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 199卷 / 01期

关键词：

D O I：

10.1006/bbrc.1994.1217

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant human leukemia inhibitory factor (rhLIF) is mitogenic for human bone-derived osteoblast-like cells in vitro. [H-3]-thymidine incorporation into DNA was stimulated dose-dependently in a prostaglandin E(2)-independent manner. rhLIF exerted no effect on either the basal or 1,25(OH)(2)D-3-induced synthesis of osteocalcin or alkaline phosphatase (AP) activity. Following treatment with rhLIF, radiolabelled cell nuclei were co-localized with AP,indicating that the mitogenic effect of rhLIF occurs on cells of the osteogenic lineage. Local generation of LIF at the human bone surface may therefore serve as a potential autocrine/paracrine mitogen for progenitor cells of the osteoblastic lineage and so could correlate with the known bone forming properties of LIF. (C) 1994 Academic Press, Inc.

引用

页码：220 / 226

页数：7

共 29 条

[1] DIFFERENTIATION-INDUCING FACTOR PURIFIED FROM CONDITIONED MEDIUM OF MITOGEN-TREATED SPLEEN-CELL CULTURES STIMULATES BONE-RESORPTION [J].

ABE, E ;

TANAKA, H ;

ISHIMI, Y ;

MIYAURA, C ;

HAYASHI, T ;

NAGASAWA, H ;

TOMIDA, M ;

YAMAGUCHI, Y ;

HOZUMI, M ;

SUDA, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (16) :5958-5962

[2]

ABE E, 1988, J BONE MINER RES, V3, P635

[3] OSTEOBLASTS DISPLAY RECEPTORS FOR AND RESPONSES TO LEUKEMIA-INHIBITORY FACTOR [J].

ALLAN, EH ;

HILTON, DJ ;

BROWN, MA ;

EVELY, RS ;

YUMITA, S ;

METCALF, D ;

GOUGH, NM ;

NG, KW ;

NICOLA, NA ;

MARTIN, TJ .

JOURNAL OF CELLULAR PHYSIOLOGY, 1990, 145 (01) :110-119

[4] THE EFFECT OF LEUKEMIA INHIBITORY FACTOR ON BONE INVIVO [J].

CORNISH, J ;

CALLON, K ;

KING, A ;

EDGAR, S ;

REID, IR .

ENDOCRINOLOGY, 1993, 132 (03) :1359-1366

[5] INCREASED POTENCY OF 1,25-DIHYDROXYVITAMIN-D3 ON HUMAN OSTEOBLAST-LIKE CELLS FOLLOWING STRUCTURAL SIDE-CHAIN MODIFICATION [J].

EVANS, DB ;

THAVARAJAH, M ;

USKOKOVIC, MR ;

KANIS, JA .

BONE, 1990, 11 (06) :439-443

[6] NATURAL HUMAN IL-1-BETA EXHIBITS REGULATORY ACTIONS ON HUMAN BONE-DERIVED CELLS-INVITRO [J].

EVANS, DB ;

BUNNING, RAD ;

VANDAMME, J ;

RUSSELL, RGG .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 159 (03) :1242-1248

[7] THE EFFECTS OF RECOMBINANT HUMAN INTERLEUKIN-1-BETA ON CELLULAR PROLIFERATION AND THE PRODUCTION OF PROSTAGLANDIN-E2, PLASMINOGEN-ACTIVATOR, OSTEOCALCIN AND ALKALINE-PHOSPHATASE BY OSTEOBLAST-LIKE CELLS DERIVED FROM HUMAN-BONE [J].

EVANS, DB ;

BUNNING, RAD ;

RUSSELL, RGG .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 166 (01) :208-216

[8] IMMUNOREACTIVITY AND PROLIFERATIVE ACTIONS OF BETA-2 MICROGLOBULIN ON HUMAN BONE-DERIVED CELLS-INVITRO [J].

EVANS, DB ;

THAVARAJAH, M ;

KANIS, JA .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 175 (03) :795-803

[9] INVOLVEMENT OF PROSTAGLANDIN-E2 IN THE INHIBITION OF OSTEOCALCIN SYNTHESIS BY HUMAN OSTEOBLAST-LIKE CELLS IN RESPONSE TO CYTOKINES AND SYSTEMIC HORMONES [J].

EVANS, DB ;

THAVARAJAH, M ;

KANIS, JA .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 167 (01) :194-202

[10]

EVANS DB, 1990, OSTEOPOROSIS, P324

← 1 2 3 →