OPTIMIZATION OF SERUM-FREE FERMENTATION PROCESSES FOR ANTIBODY-PRODUCTION

被引：58

作者：

BUNTEMEYER, H

LUTKEMEYER, D

LEHMANN, J

机构：

[1] Institute for Cell Culture Technique, University of Bielefeld, Bielefeld 1, 4800

来源：

CYTOTECHNOLOGY | 1991年 / 5卷 / 01期

关键词：

SERUM-FREE MEDIUM; ANTIBODY PRODUCTION; HYBRIDOMA; AMINO ACID ANALYSIS; SUBSTRATE; UTILIZATION;

D O I：

10.1007/BF00365534

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Serum free fermentation procedures of cell cultures have got a wide application in production of biochemicals. But, cells cultured in serum free media in general are more sensitive to changes in culture condition, especially to nutrient limitation. There are no substances from serum which can support the cells when conditions are changing. In this study special attention is directed to amino acid utilization of mouse hybridoma in batch, chemostat and perfusion fermentations. Detailed data are presented which show the considerable difference of amino acid consumption rates in different fermentation modes. Already, in batch mode there are differences of the two investigated mouse hybridoma cell lines, although they are derived from the same myeloma line. In chemostat running at a dilution rate representing maximal growth rate most of the consumption rates are significant higher than in batch. On the other hand, in perfusion mode the rates are lower than in batch. This indicates clearly the different conditions of the fermentation modes. Therefore, it is necessary to develop serum free processes under the desired production conditions. An accurate analysis of the process is strongly recommended.

引用

页码：57 / 67

页数：11

共 8 条

[1]

Bober H., Über Möglichkeiten der HPLC zur Trennung und Bestimmung von Aminosäuren I, Trennung von Hydrolysatgemischen Beckman Report, 1986, (1986)

[2]

Buntemeyer H., Entwicklung eines Perfusionssystems zur kontinuierlichen Kultivierung tierischer Zellen in Suspension, Ph.D. Thesis, (1988)

[3]

Eagle H., Amino acid metabolism in mammalian cell cultures, Science, 130, pp. 432-437, (1959)

[4]

Geaugey V., Duval D., Geahel I., Marc A., Engasser J.M., Influence of amino acids on hybridoma cell viability and antibody secretion, Cytotechnology, 2, pp. 119-129, (1989)

[5]

Jager V., Lehmann J., Friedl P., Serum-free growth medium for the cultivation of a wide spectrum of mammalian cells in stirred bioreactors, Cytotechnology, 1, pp. 319-329, (1988)

[6]

Kohler G., Milstein C., Continuous cultures of fused cells secreting antibody of predefined specifity, Nature, 256, pp. 495-497, (1975)

[7]

Takazawa Y., Tokashiki M., Murakami H., Yamada K., Omura H., High-density culture of mouse-human hybridoma in serum-free defined medium, Biotechnol. Bioeng., 31, pp. 168-172, (1988)

[8]

Wagner R., Ryll T., Krafft H., Lehmann J., Variation of amino acid concentrations in the medium of HU β-IFN and HU IL-2 producing cell lines, Cytotechnology, 1, pp. 145-150, (1988)

← 1 →