GENOMIC ORGANIZATION OF THE HALOPHILIC ARCHAEON HALOFERAX-MEDITERRANEI - PHYSICAL MAP OF THE CHROMOSOME

被引：29

作者：

LOPEZGARCIA, P

ABAD, JP

SMITH, C

AMILS, R

机构：

[1] UNIV AUTONOMA MADRID, CTR BIOL MOLEC, E-28049 MADRID, SPAIN

[2] LAWRENCE BERKELEY LAB, DEPT MOLEC & CELL BIOL, BERKELEY, CA 94720 USA

[3] LAWRENCE BERKELEY LAB, DIV CHEM BIODYNAM, BERKELEY, CA 94720 USA

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 10期

关键词：

D O I：

10.1093/nar/20.10.2459

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pulsed field gel electrophoresis (PFG) has been used to study the genomic organization of the halophilic archaeon Haloferax mediterranei. Analysis of the different genomic elements as well as the restriction patterns obtained with several endonucleases revealed that this microorganism has a circular chromosome of 2.9 Mb and, at least, three extrachromosomal elements of 490, 320 and 130 kb respectively. The complete physical map of the chromosome for the endonucleases PacI and BamHI has been constructed, and several BcII, BgIII and DraI restriction fragments have been aligned on these maps. The localization of heterologous and homologous genes on the physical map, including those for rRNA, lay the ground work for the construction of a genetic map.

引用

页码：2459 / 2464

页数：6

共 30 条

[1]

ABAD JP, 1991, GENERAL APPLIED ASPE, P295

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