LOCALIZATION OF HYDROGEN-PEROXIDE PRODUCTION IN PISUM-SATIVUM L USING EPI-POLARIZATION MICROSCOPY TO FOLLOW CERIUM PERHYDROXIDE DEPOSITION

被引：18

作者：

LIU, L ^{[1
]}

ERIKSSON, KEL ^{[1
]}

DEAN, JFD ^{[1
]}

机构：

[1] UNIV GEORGIA, CTR BIOL RESOURCE RECOVERY, DEPT BIOCHEM & MOLEC BIOL, ATHENS, GA 30602 USA

来源：

PLANT PHYSIOLOGY | 1995年 / 107卷 / 02期

关键词：

D O I：

10.1104/pp.107.2.501

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Cerium is becoming an increasingly popular reagent for histochemical localization of oxidases and phosphatases because it combines directly with reaction products to form fine precipitates of electron-dense materials that can be easily detected using transmission electron microscopy or laser confocal scanning microscopy. We used epi-polarization microscopy to detect cerium perhydroxide deposits formed when H2O2 was produced by diamine oxidase in pea (Pisum sativum L.) epicotyls exposed to exogenous putrescine. Diamine oxidase activity was abundant in cortical cell walls but showed little, if any, association with vascular tissues. Maps of cerium deposition generated using scanning electron microscopy/x-ray microanalysis verified these observations. This study demonstrates the use of epi-polarization microscopy to follow cerium deposition, and the ready accessibility of this microscopy technique should facilitate more widespread use of cerium for plant histochemistry and cytochemistry.

引用

页码：501 / 506

页数：6

共 38 条

[1] HISTOCHEMICAL EVIDENCE OF POLYAMINE OXIDATION AND GENERATION OF HYDROGEN-PEROXIDE IN THE CELL-WALL [J].

ANGELINI, R ;

FEDERICO, R .

JOURNAL OF PLANT PHYSIOLOGY, 1989, 135 (02) :212-217

[2] ELICITOR-INDUCED AND WOUND-INDUCED OXIDATIVE CROSS-LINKING OF A PROLINE-RICH PLANT-CELL WALL PROTEIN - A NOVEL, RAPID DEFENSE RESPONSE [J].

BRADLEY, DJ ;

KJELLBOM, P ;

LAMB, CJ .

CELL, 1992, 70 (01) :21-30

[3] LOCALIZATION OF NADH OXIDASE ON SURFACE OF HUMAN POLYMORPHONUCLEAR LEUKOCYTES BY A NEW CYTOCHEMICAL METHOD [J].

BRIGGS, RT ;

DRATH, DB ;

KARNOVSKY, ML ;

KARNOVSKY, MJ .

JOURNAL OF CELL BIOLOGY, 1975, 67 (03) :566-586

[4] AUXIN-STIMULATED NADH OXIDASE PURIFIED FROM PLASMA-MEMBRANE OF SOYBEAN [J].

BRIGHTMAN, AO ;

BARR, R ;

CRANE, FL ;

MORRE, DJ .

PLANT PHYSIOLOGY, 1988, 86 (04) :1264-1269

[5] CYTOCHEMICAL-LOCALIZATION OF PLASMA-MEMBRANE ATPASE ACTIVITY IN PLANT-CELLS - A COMPARISON OF LEAD AND CERIUM-BASED METHODS [J].

CHAUHAN, E ;

COWAN, DS ;

HALL, JL .

PROTOPLASMA, 1991, 165 (1-3) :27-36

[6] ACTIVE OXYGEN SPECIES IN THE INDUCTION OF PLANT SYSTEMIC ACQUIRED-RESISTANCE BY SALICYLIC-ACID [J].

CHEN, ZX ;

SILVA, H ;

KLESSIG, DF .

SCIENCE, 1993, 262 (5141) :1883-1886

[7] 4-COUMARATE, COENZYME-A LIGASE AND ISOPEROXIDASE EXPRESSION IN ZINNIA MESOPHYLL-CELLS INDUCED TO DIFFERENTIATE INTO TRACHEARY ELEMENTS [J].

CHURCH, DL ;

GALSTON, AW .

PLANT PHYSIOLOGY, 1988, 88 (03) :679-684

[8] CROSS-LINKING OF SOLUBLE EXTENSIN IN ISOLATED CELL-WALLS [J].

COOPER, JB ;

VARNER, JE .

PLANT PHYSIOLOGY, 1984, 76 (02) :414-417

[9]

DEAN JFD, 1992, HOLZFORSCHUNG, V46, P135

[10]

DEMEY H, 1983, IMMUNOCYTOCHEMISTRY, P82

← 1 2 3 4 →