COMPARISON OF 3-DIMENSIONAL IMAGING PROPERTIES BETWEEN 2-PHOTON AND SINGLE-PHOTON FLUORESCENCE MICROSCOPY

被引：82

作者：

GU, M

SHEPPARD, CJR

机构：

[1] Department of Physical Optics, University of Sydney, New South Wales

来源：

JOURNAL OF MICROSCOPY-OXFORD | 1995年 / 177卷

关键词：

CONFOCAL MICROSCOPY; 2-PHOTON FLUORESCENCE IMAGING; 3-DIMENSIONAL IMAGING; IMAGE FORMATION; FLUORESCENCE MICROSCOPY; 4PI CONFOCAL MICROSCOPY;

D O I：

10.1111/j.1365-2818.1995.tb03543.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

The imaging performance in single-photon (1-p) and two-photon (2-p) fluorescence microscopy is described. Both confocal and conventional systems are compared in terms of the three-dimensional (3-D) point spread function and the 3-D optical transfer function. Images of fluorescent sharp edges and layers are modelled, giving resolution in transverse and axial directions. A comparison of the imaging properties is also given for a 4Pi confocal system, Confocal 2-p 4Pi fluorescence microscopy gives the best axial resolution in the sense that its 3-D optical transfer function has the strongest response along the axial direction.

引用

页码：128 / 137

页数：10

共 31 条

[1] Denk W., Strickler J.H., Webb W.W., Two‐photon laser scanning fluorescence microscopy, Science, 248, pp. 73-76, (1990)
[2] Frieden B.R., Optical transfer of the three‐dimensional object, Journal of the Optical Society of America, 57, pp. 56-66, (1967)
[3] Gannaway J., Sheppard C.J.R., Second harmonic imaging in the scanning optical microscope, Opt. Quant. Elec., 10, pp. 435-439, (1978)
[4] Gu, Sheppard C.J.R., Effects of a finite‐sized detector on the OTF of confocal fluorescent microscopy, Optik, 89, pp. 65-69, (1991)
[5] Gu, Sheppard C.J.R., Confocal fluorescence microscopy with a finite‐sized circular detector, Journal of the Optical Society of America A, 9, pp. 151-153, (1992)
[6] Gu, Sheppard C.J.R., Effects of a finite‐sized pinhole on 3D image formation in confocal two‐photon fluorescence microscopy, J. Mol. Opt., 40, pp. 2009-2024, (1993)
[7] Gu, Sheppard C.J.R., Three‐dimensional transfer functions in 4Pi confocal microscopes, J. Opt. Soc. Am. A, 11, pp. 1619-1627, (1994)
[8] Gu, Tannous T., Sheppard C.J.R., Improved axial resolution in confocal fluorescence microscopy using annular pupils, Opt. Commun., 110, pp. 533-539, (1994)
[9] Hell S.W., Lindek S., Stelzer E.H.K., Enhancing the axial resolution in far‐field light microscopy: two‐photon 4Pi‐confocal fluorescence microscopy, Journal of Modern Optics, 41, pp. 675-681, (1994)
[10] Hell S.W., Stelzer E.H.K., Properties of a 4Pi confocal fluorescence microscope, Journal of the Optical Society of America A, 9, pp. 2159-2166, (1992)

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