ACINETOBACTER-CALCOACETICUS LIBERATES CHROMOSOMAL DNA DURING INDUCTION OF COMPETENCE BY CELL-LYSIS

被引：24

作者：

PALMEN, R ^{[1
]}

HELLINGWERF, KJ ^{[1
]}

机构：

[1] BIOCENTRUM AMSTERDAM,EC SLATER INST BIOCHEM RES,DEPT MICROBIOL,1018 WS AMSTERDAM,NETHERLANDS

来源：

CURRENT MICROBIOLOGY | 1995年 / 30卷 / 01期

关键词：

D O I：

10.1007/BF00294516

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A transformation assay was used to assay the amount of DNA present in the extracellular medium of a growing culture of Acinetobacter calcoaceticus. It was observed that small amounts of DNA were liberated during the entire exponential growth phase in a batch culture. Release of DNA could be fully accounted for by lysis of cells. Lysis was quantified via simultaneous measurement of beta-galactosidase activity of cells and supernatant, with a strain that contained a plasmid (pAPA100) with lacZ under control of a constitutive beta-lactamase promoter. In conclusion, no evidence could be obtained indicating that Acinetobacter calcoaceticus actively excretes DNA, to be used for DNA exchange.

引用

页码：7 / 10

页数：4

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