ANALYSIS OF PBR322 REPLICATION KINETICS AND ITS DEPENDENCY ON GROWTH-RATE

被引:31
作者
KIM, BG
SHULER, ML
机构
[1] CORNELL UNIV,DEPT CHEM ENGN,ITHACA,NY 14853
[2] CORNELL UNIV,DEPT FOOD SCI & TECHNOL,ITHACA,NY 14853
关键词
D O I
10.1002/bit.260360304
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Accurate estimates of plasmid copy number in a cell are a prerequisite for predicting plasmid stability and protein production. A refined version of a structured model for the pBR322 plasmid replication mechanism is described. The model is capable of accurately predicting pBR322 plasmid copy number in Escherichia coli B/r for a wide range of growth rates. The refinements include better estimates of promoter strength, the degradation rate of RNA species, binding constant of RNAI–RNAII reaction, and dependency of promoter strength on growth rate. The predictions of the model are verified by recent experimental observations but differ from some previous reports. This model can also be used to predict the binding constant of the RNAI–RNAII reaction of ColE1 type plasmids. At 37°C, the binding constant is estimated to be 77 ± 11 × 10−13 mL/molecule‐h for pBR322. Copyright © 1990 John Wiley & Sons, Inc.
引用
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页码:233 / 242
页数:10
相关论文
共 33 条
[1]   A MATHEMATICAL-MODEL FOR PREDICTION OF PLASMID COPY NUMBER AND GENETIC STABILITY IN ESCHERICHIA-COLI [J].
ATAAI, MM ;
SHULER, ML .
BIOTECHNOLOGY AND BIOENGINEERING, 1987, 30 (03) :389-397
[2]   MATHEMATICAL-MODEL FOR THE CONTROL OF COLE1 TYPE PLASMID REPLICATION [J].
ATAAI, MM ;
SHULER, ML .
PLASMID, 1986, 16 (03) :204-212
[3]   CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .2. MULTIPURPOSE CLONING SYSTEM [J].
BOLIVAR, F ;
RODRIGUEZ, RL ;
GREENE, PJ ;
BETLACH, MC ;
HEYNEKER, HL ;
BOYER, HW ;
CROSA, JH ;
FALKOW, S .
GENE, 1977, 2 (02) :95-113
[4]   PLASMID REPLICATION FUNCTIONS .8. REPLICATION CONTROL MUTATIONS OF PLASMID R6-5 AND THEIR EFFECTS ON INTERACTIONS OF THE RNA-I CONTROL ELEMENT WITH ITS TARGET [J].
BRADY, G ;
FREY, J ;
DANBARA, H ;
TIMMIS, KN .
JOURNAL OF BACTERIOLOGY, 1983, 154 (01) :429-436
[5]   CONTROL OF PMB1 REPLICATION - INHIBITION OF PRIMER FORMATION BY ROP REQUIRES RNA1 [J].
CESARENI, G ;
CORNELISSEN, M ;
LACATENA, RM ;
CASTAGNOLI, L .
EMBO JOURNAL, 1984, 3 (06) :1365-1369
[6]   CONTROL OF COLE1 DNA-REPLICATION - THE ROP GENE-PRODUCT NEGATIVELY AFFECTS TRANSCRIPTION FROM THE REPLICATION PRIMER PROMOTER [J].
CESARENI, G ;
MUESING, MA ;
POLISKY, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (20) :6313-6317
[7]  
CHAO SL, 1987, J BACTERIOL, V169, P1217
[8]  
CHAO SL, 1986, J THEOR BIOL, V125, P453
[9]  
CHAO SL, 1986, MOL GEN GENET, V203, P143
[10]  
CHAO SL, 1986, MOL GEN GENET, V203, P150