A G-BETA PROTEIN IN THE DROSOPHILA COMPOUND EYE IS DIFFERENT FROM THAT IN THE BRAIN

被引：61

作者：

YARFITZ, S ^{[1
]}

NIEMI, GA ^{[1
]}

MCCONNELL, JL ^{[1
]}

FITCH, CL ^{[1
]}

HURLEY, JB ^{[1
]}

机构：

[1] UNIV WASHINGTON,DEPT BIOCHEM,SEATTLE,WA 98195

来源：

NEURON | 1991年 / 7卷 / 03期

关键词：

D O I：

10.1016/0896-6273(91)90295-B

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A G protein-beta-subunit gene (Gbe) is expressed only in the eyes of adult D. melanogaster. This gene was identified by probing a Drosophila head cDNA expression library with monoclonal antibodies to a previously characterized Drosophila G protein-beta-subunit (Gbb). Immunoblot and Northern analyses demonstrate that Gbe protein and mRNA is not present in Drosophila mutants that lack eyes. Immunocytochemical and in situ hybridization analyses further demonstrate that Gbe is expressed in the eyes but not in the brain, whereas Gbb is abundantly expressed in the brain. The Gbe product is approximately 45% identical to previously identified G-beta-subunits and defines a new G-beta-class. Its localization suggests a possible role in phototransduction.

引用

页码：429 / 438

页数：10

共 65 条

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