EXCRETION AND RAPID PURIFICATION OF GAMMA-GLUTAMYL-TRANSPEPTIDASE FROM ESCHERICHIA-COLI K-12

被引：17

作者：

CLAUDIO, JO ^{[1
]}

SUZUKI, H ^{[1
]}

KUMAGAI, H ^{[1
]}

TOCHIKURA, T ^{[1
]}

机构：

[1] KYOTO UNIV,FAC AGR,DEPT FOOD SCI & TECHNOL,SAKYO KU,KYOTO 606,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1991年 / 72卷 / 02期

关键词：

D O I：

10.1016/0922-338X(91)90322-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

tolA strains of Escherichia coli harboring plasmids carrying the ggt gene excreted about 70% of their gamma-glutamyltranspeptidase (GGT) (EC 2.3.2.2) into the medium. The excreted GGT was purified to electrophoretic homogeneity by a simple two-step method with a yield of 51%.

引用

页码：125 / 127

页数：3

共 25 条

[1]

ABRAHAMSEN L, 1982, NUCLEIC ACIDS RES, V14, P7487

[2] DEFECTIVE TRANSPORT AND OTHER PHENOTYPES OF A PERIPLASMIC LEAKY MUTANT OF ESCHERICHIA-COLI-K-12 [J].

ANDERSON, JJ ;

WILSON, JM ;

OXENDER, DL .

JOURNAL OF BACTERIOLOGY, 1979, 140 (02) :351-358

[3] RELEASE OF THERMOPHILIC ALPHA-AMYLASE FROM TRANSFORMED ESCHERICHIA-COLI BY ADDITION OF GLYCINE [J].

ARIGA, O ;

WATARI, T ;

ANDOH, Y ;

FUJISHITA, Y ;

SANO, Y .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1989, 68 (04) :243-246

[4] LINKAGE MAP OF ESCHERICHIA-COLI-K-12, EDITION-7 [J].

BACHMANN, BJ .

MICROBIOLOGICAL REVIEWS, 1983, 47 (02) :180-230

[5] POSITIVE SELECTION FOR LOSS OF TETRACYCLINE RESISTANCE [J].

BOCHNER, BR ;

HUANG, HC ;

SCHIEVEN, GL ;

AMES, BN .

JOURNAL OF BACTERIOLOGY, 1980, 143 (02) :926-933

[6] COLONY BANK CONTAINING SYNTHETIC COL EL HYBRID PLASMIDS REPRESENTATIVE OF ENTIRE ESCHERICHIA-COLI GENOME [J].

CLARKE, L ;

CARBON, J .

CELL, 1976, 9 (01) :91-99

[7]

CSNOKA LN, 1980, J BACTERIOL, V143, P529

[8] TOLA, TOLB AND EXCC, 3 CISTRONS INVOLVED IN THE CONTROL OF PLEIOTROPIC RELEASE OF PERIPLASMIC PROTEINS BY ESCHERICHIA-COLI-K12 [J].

FOGNINILEFEBVRE, N ;

LAZZARONI, JC ;

PORTALIER, R .

MOLECULAR & GENERAL GENETICS, 1987, 209 (02) :391-395

[9] EFFECT OF GLYCINE AND ITS DERIVATIVES ON PRODUCTION AND RELEASE OF BETA-GALACTOSIDASE BY ESCHERICHIA-COLI [J].

IKURA, Y .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1986, 50 (11) :2747-2753

[10] A CONTROLLABLE EXPRESSION-SECRETION VECTOR CONSTRUCTED FROM THE MULTIPLE TRP PROMOTER-OPERATOR, THE SIGNAL PEPTIDE REGION OF THE OMPF GENE AND THE TRPR GENE IN ESCHERICHIA-COLI [J].

ITOH, M ;

AIBA, H ;

INOKUCHI, K ;

MIZUNO, T ;

NAGAHARI, K ;

MUNAKATA, K ;

MIZUSHIMA, S .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1986, 50 (05) :1295-1302

← 1 2 3 →