DETECTION OF BORRELIACIDAL ANTIBODIES IN HAMSTERS BY USING FLOW-CYTOMETRY

被引：3

作者：

SACHSENMEIER, KF

SCHELL, K

MORRISSEY, LW

PENNELL, DR

WEST, RM

CALLISTER, SM

SCHELL, RF

机构：

[1] UNIV WISCONSIN, WISCONSIN STATE LAB HYG, MADISON, WI 53706 USA

[2] UNIV WISCONSIN, DEPT MED MICROBIOL & IMMUNOL, MADISON, WI 53706 USA

[3] UNIV WISCONSIN, CTR COMPREHENS CANC, FLOW CYTOMETRY UNIT, MADISON, WI 53706 USA

[4] GUNDERSON MED FDN, LA CROSSE, WI 54601 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1992年 / 30卷 / 06期

关键词：

D O I：

10.1128/JCM.30.6.1457-1461.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Flow cytometry can be used to detect antibody that kills Borrelia burgdorferi. Borreliacidal activity was detected within 3 h of incubating B. burgdorferi with immune serum and complement. Right-angle light scatter and propidium iodide fluorescence were the cytometric parameters which correlated best with in vitro killing of B. burgdorferi. Flow cytometry is a rapid method for determining the presence of borreliacidal activity and may lead to a better serodiagnostic test for the detection of Lyme disease.

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页码：1457 / 1461

页数：5

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