ELECTRICAL-PROPERTIES OF THE ISOLATED, IN-VITRO PERFUSED MALPIGHIAN TUBULE OF THE ANT, THE CL- PATHWAY

被引：13

作者：

DIJKSTRA, S ^{[1
]}

LOHRMANN, E ^{[1
]}

STEELS, P ^{[1
]}

GREGER, R ^{[1
]}

机构：

[1] UNIV FREIBURG,INST PHYSIOL,W-7800 FREIBURG,GERMANY

来源：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 1994年 / 4卷 / 1-2期

关键词：

MALPIGHIAN TUBULES; CL--PATHWAY; SHUNT SELECTIVITY; MEMBRANE RESISTANCES;

D O I：

10.1159/000154706

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

An electrical characterization of the isolated, perfused malpighian tubule of Formica polyctena and F. rufa is reported. Some specific problems of the perfusion method as applied to malpighian tubules of the ant are discussed and a practical solution presented. Estimates for the individual membrane resistances are derived. The shunt is investigated by basolateral biionic replacements and found to be anion specific. The Cl- pathway is further investigated by the addition of Cl- channel blockers, 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB) and 4,4'-diisothiocyano-2,2'-stilbenedisulfonate (DIDS), to the lumen. The lack of effect suggests the absence of DIDS- and NPPB-sensitive Cl- channels.

引用

页码：19 / 30

页数：12

共 15 条

[1] MICROELECTRODE STUDIES ON MALPIGHIAN TUBULE CELLS OF LOCUSTA-MIGRATORIA - EFFECTS OF EXTERNAL IONS AND INHIBITORS [J].