RATES OF EXCHANGE OF MITOCHONDRIAL MG2+ DETERMINED FROM 28MG FLUX MEASUREMENTS

被引：41

作者：

JOHNSON, JH

PRESSMAN, BC

机构：

[1] Johnson Research Foundation, Department of Biophysics and Physical Biochemistry, University of Pennsylvania, Philadelphia

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1969年 / 132卷 / 01期

关键词：

D O I：

10.1016/0003-9861(69)90345-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The rate of exchange of the endogenous Mg2+ of isolated rat liver mitochondria was measured by means of the radioisotope 28Mg. Two different centrifugation techniques yielding similar results, were utilized for separating the mitochondria from suspenpension media for analysis. Mitochondrial Mg2+ exchanges very slowly with externally added Mg2+. The influx of labeled Mg2+ observed in the presence of substrate is blocked by treatment with the respiratory inhibitor, rotenone, or the uncoupler, p-trifluoromethoxy(carbonylcyanide) phenylhydrazone. The observed rates of Mg2+ influx are similar in order of magnitude to rates of K+ influx measured under comparable conditions. Parathyroid hormone stimulates Mg2+ influx, but only when metabolic energy is available to support net Mg2+ uptake. © 1969.

引用

页码：139 / &

共 24 条

[1] PERMEABILITY OF ISOLATED RAT-LIVER MITOCHONDRIA TO SUCROSE, SODIUM CHLORIDE AND POTASSIUM CHLORIDE AT 0-DEGREES
AMOORE, JE
BARTLEY, W
[J]. BIOCHEMICAL JOURNAL, 1958, 69 : 223 - 236
[2] AUTOMATIC LIQUID SCINTILLATION COUNTING OF HIGH-ENERGY BETA EMITTERS IN TISSUE SLICES AND AQUEOUS SOLUTIONS IN ABSENCE OF ORGANIC SCINTILLATOR
BRAUNSBERG, H
GUYVER, A
[J]. ANALYTICAL BIOCHEMISTRY, 1965, 10 (01) : 86 - +
[3] A SIMPLE EFFICIENT LIQUID SCINTILLATOR FOR COUNTING AQUEOUS SOLUTIONS IN A LIQUID SCINTILLATION COUNTER
BRAY, GA
[J]. ANALYTICAL BIOCHEMISTRY, 1960, 1 (4-5) : 279 - 285
[4] BRIERLEY G, 1963, J BIOL CHEM, V238, P3482
[5] BRIERLEY GP, 1967, J BIOL CHEM, V242, P1115
[6] CARAFOLI E, 1964, J BIOL CHEM, V239, P3055
[7] TRANSIENT PERMEABILITY CHANGES OF MITOCHONDRIA INDUCED BY UNCOUPLING AGENTS
CASWELL, AH
PRESSMAN, BC
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1968, 30 (06) : 637 - &
[8] SYNTHESIS OF ATP DRIVEN BY A POTASSIUM GRADIENT IN MITOCHONDRIA
COCKRELL, RS
HARRIS, EJ
PRESSMAN, BC
[J]. NATURE, 1967, 215 (5109) : 1487 - &
[9] RETENTION OF POTASSIUM BY MITOCHONDRIA
GAMBLE, JL
[J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1962, 203 (05): : 886 - &
[10] EFFECT OF TRANSPORT-INDUCING ANTIBIOTICS AND OTHER AGENTS ON POTASSIUM FLUX IN MITOCHONDRIA
HARRIS, EJ
CATLIN, G
PRESSMAN, BC
[J]. BIOCHEMISTRY, 1967, 6 (05) : 1360 - &

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