NMR-STUDIES OF THE INTERACTION OF BLEOMYCIN WITH (DC-DG)3

被引：13

作者：

GAMCSIK, MP ^{[1
]}

GLICKSON, JD ^{[1
]}

ZON, G ^{[1
]}

机构：

[1] APPL BIOSYST INC,FOSTER CITY,CA 94494

来源：

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS | 1990年 / 7卷 / 05期

关键词：

D O I：

10.1080/07391102.1990.10508550

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction of bleomycin A2 and Zn(II)-bleomycin A2 with the oligonucleotide (dC-dG)3 has been monitored by nuclear magnetic resonance spectroscopy. Binding of the drug to the oligonucleotide is indicated by an upheld shift of the bithiazole proton resonances consistent with partial intercalation of this group between base pairs. The effect of temperature and ionic strength on the binding of both free bleomycin and the Zn(II) complex has been studied. Consistent with earlier studies on polynucleotides, the rate of exchange between the free drug and the drug-oligonucleotide complex is rapid on the1H NMR chemical shift time scale. Binding of the oligonucleotide induced changes in resonances assigned to protons in the metal-binding region of Zn(II)-bleomycin. Intermolecular nuclear Overhauser effect enhancements between bleomycin and the oligonucleotide have not been detected. © Taylor & Francis Group, LLC.

引用

收藏

页码：1117 / 1133

页数：17

相关论文

共 40 条

[1] STUDIES OF THE SOLUTION STRUCTURE OF THE BLEOMYCIN-A2 ZINC COMPLEX BY MEANS OF TWO-DIMENSIONAL NMR-SPECTROSCOPY AND DISTANCE GEOMETRY CALCULATIONS [J].

AKKERMAN, MAJ ;

HAASNOOT, CAG ;

HILBERS, CW .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 173 (01) :211-225

[2] PRACTICAL ASPECTS OF TWO-DIMENSIONAL TRANSVERSE NOE SPECTROSCOPY [J].

BAX, A ;

DAVIS, DG .

JOURNAL OF MAGNETIC RESONANCE, 1985, 63 (01) :207-213

[3] INTERACTION OF BLEOMYCIN-A2 WITH POLY(DEOXYADENYLYLTHYMIDYLIC ACID) - A PROTON NUCLEAR MAGNETIC-RESONANCE STUDY OF THE INFLUENCE OF TEMPERATURE, PH, AND IONIC-STRENGTH [J].

BOOTH, TE ;

SAKAI, TT ;

GLICKSON, JD .

BIOCHEMISTRY, 1983, 22 (18) :4211-4217

[4] STRUCTURE DETERMINATION OF A TETRASACCHARIDE - TRANSIENT NUCLEAR OVERHAUSER EFFECTS IN THE ROTATING FRAME [J].

BOTHNERBY, AA ;

STEPHENS, RL ;

LEE, JM ;

WARREN, CD ;

JEANLOZ, RW .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1984, 106 (03) :811-813

[5] PROTON NUCLEAR MAGNETIC-RESONANCE STUDY OF BLEOMYCIN IN AQUEOUS-SOLUTION - ASSIGNMENT OF RESONANCES [J].

CHEN, DM ;

HAWKINS, BL ;

GLICKSON, JD .

BIOCHEMISTRY, 1977, 16 (12) :2731-2738

[6] BLEOMYCIN-A2 COMPLEXES WITH POLY(DA-DT) - PROTON NUCLEAR MAGNETIC-RESONANCE STUDY OF THE NON-EXCHANGEABLE HYDROGENS [J].

CHEN, DM ;

SAKAI, TT ;

GLICKSON, JD ;

PATEL, DJ .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1980, 92 (01) :197-205

[7] H-1-NUCLEAR AND P-31 NUCLEAR MAGNETIC-RESONANCE STUDIES ON THE CONFORMATION OF D-(CPGPCPG)2 AND D-(CPGPCPGPCPG)2 SHORT HELICES IN B-CONFORMATION [J].

CHENG, DM ;

KAN, LS ;

FRECHET, D ;

TSO, POP ;

UESUGI, S ;

SHIDA, T ;

IKEHARA, M .

BIOPOLYMERS, 1984, 23 (04) :775-795

[8] BLEOMYCIN-DNA INTERACTIONS - FLUORESCENCE AND PROTON MAGNETIC-RESONANCE STUDIES [J].

CHIEN, M ;

GROLLMAN, AP ;

HORWITZ, SB .

BIOCHEMISTRY, 1977, 16 (16) :3641-3647

[9] TRANSITION-METAL BINDING-SITE OF BLEOMYCIN A2 - C-13 NUCLEAR MAGNETIC-RESONANCE STUDY OF ZINC(II) AND COPPER(II) DERIVATIVES [J].

DABROWIAK, JC ;

GREENAWAY, FT ;

GRULICH, R .

BIOCHEMISTRY, 1978, 17 (19) :4090-4096

[10] SEQUENCE SPECIFIC CLEAVAGE OF DNA BY ANTI-TUMOR ANTIBIOTICS NEOCARZINOSTATIN AND BLEOMYCIN [J].

DANDREA, AD ;

HASELTINE, WA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (08) :3608-3612

← 1 2 3 4 →