DUAL EFFECTS OF SUPEROXIDE-DISMUTASE ON THE AUTOXIDATION OF 1,4-NAPHTHOHYDROQUINONE

被引：43

作者：

ISHII, T ^{[1
]}

FRIDOVICH, I ^{[1
]}

机构：

[1] DUKE UNIV,MED CTR,DEPT BIOCHEM,DURHAM,NC 27710

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 1990年 / 8卷 / 01期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

Autoxidation of superoxide dismutase; Effect on hydroquinone oxidation autocatalysis; Free radicals; Hydroquinones; In hydroquinone autoxidation superoxide radical; Propagates hydroquinone autoxidation;

D O I：

10.1016/0891-5849(90)90140-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The autoxidation of 1,4-naphthohydroquinone, in a phosphate, EDTA buffer at pH 7.4, exhibits an autocatalysis whose lag phase becomes more pronounced in the presence of either the Cu,Zn- or the Mn-containing superoxide dismutases. In contrast, the autoxidation of a second aliquot of the hydroquinone, added after complete oxidation of the first, is linear and is accelerated by superoxide dismutase. Catalase or inactive superoxide dismutase were without effect in either situation. These results are explicable in terms of a free radical chain reaction which is initially propagated by O2- and then, as the quinone accumulates, by univalent reduction of the quinone by the hydroquinone. Reduction of the quinone by O2- diminishes the overall rate of oxidation. It is not necessary to postulate catalysis by superoxide dismutase of the reduction of the semiquinone by O2-. © 1990.

引用

页码：21 / 24

页数：4

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