EVIDENCE THAT A PROTEIN-KINASE ENHANCES AMSACRINE MEDIATED FORMATION OF TOPOISOMERASE II-DNA COMPLEXES IN MURINE MASTOCYTOMA CELL-NUCLEI

被引：12

作者：

DARKINRATTRAY, SJ ^{[1
]}

RALPH, RK ^{[1
]}

机构：

[1] UNIV AUCKLAND,DEPT CELLULAR & MOLEC BIOL,AUCKLAND,NEW ZEALAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1088卷 / 02期

关键词：

AMSACRINE; TOPOISOMERASE II-DNA COMPLEX FORMATION ENHANCING FACTOR; CASEIN KINASE-II; NUCLEUS; (MURINE MASTOCYTOMA CELL);

D O I：

10.1016/0167-4781(91)90065-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytoplasmic extracts of K21 murine mastocytoma cells contain a protein factor, distinct from topoisomerases I and II, that facilitates formation of amsacrine-induced topoisomerase II-DNA complexes (PDC) in isolated K21 cell nuclei (Darkin, S.J. and Ralph, R.K. (1988) Biochim. Biophys. Acta 1007, 295-300). The PDC enhancing activity was shown to reside in a protein kinase with specificity for a casein kinase II substrate and sensitive to heparin and anti-casein kinase II antiserum. This appears to be the first direct evidence of a protein factor that modulates amsacrine-induced topoisomerase II action.

引用

页码：285 / 291

页数：7

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