EFFECT OF ZINC IONS ON THE BIOCHEMICAL BEHAVIOR OF SIMIAN VIRUS-40 SMALL-T ANTIGEN EXPRESSED IN BACTERIA

被引：17

作者：

GOSWAMI, R

TURK, B

ENDERLE, K

HOWE, A

RUNDELL, K

机构：

[1] NORTHWESTERN UNIV, DEPT MICROBIOL IMMUNOL, 303 E CHICAGO AVE, CHICAGO, IL 60611 USA

[2] NORTHWESTERN UNIV, CTR CANC, CHICAGO, IL 60611 USA

来源：

JOURNAL OF VIROLOGY | 1992年 / 66卷 / 03期

关键词：

D O I：

10.1128/JVI.66.3.1746-1751.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The simian virus 40 small-t antigen contains 10 cysteine residues, 6 of which are organized in two CysXCysXXCys clusters. Mutation of individual Cys residues in the two clusters or mutation of specific residues found between these clusters causes pronounced instability of the protein in animal cells. Protein instability correlates with failure of the bacterially expressed mutant proteins to bind zinc ions, an interaction which allows purification of large amounts of small-t antigen in monomeric form.

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页码：1746 / 1751

页数：6

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