CYP52 (CYTOCHROME-P450ALK) MULTIGENE FAMILY IN CANDIDA-MALTOSA - MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF THE 2 TANDEMLY ARRANGED GENES

被引：52

作者：

OHKUMA, M ^{[1
]}

TANIMOTO, T ^{[1
]}

YANO, K ^{[1
]}

TAKAGI, M ^{[1
]}

机构：

[1] UNIV TOKYO,DEPT AGR CHEM,BUNKYO KU,TOKYO 113,JAPAN

来源：

DNA AND CELL BIOLOGY | 1991年 / 10卷 / 04期

关键词：

D O I：

10.1089/dna.1991.10.271

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Southern blot analysis under low-stringency conditions using a previously isolated n-alkane-inducible cytochrome P450 (P450alk) gene as a probe revealed the presence of multiple P450alk-related genes in the genome of Candida maltosa. Nine P450alk-related genes (one reported previously and eight in the present report) were isolated from a genomic library constructed from this strain, and these were classified on the basis of sequence similarities into three pairs of putative allelic genes and three nonallelic genes. Two pairs of these alleles were tandemly arranged in the genome. The complete nucleotide sequences of one of these pairs were determined and compared to other members of this P450 family (CYP52) in C. maltosa and C. tropicalis. Northern blot analysis further showed that these genes were regulated by carbon sources. These results provide evidence for a P450alk (CYP52) multigene family in C. maltosa.

引用

页码：271 / 282

页数：12

共 43 条

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