DETECTION OF STABLE ACETALDEHYDE-MODIFIED PROTEINS IN THE LIVERS OF ETHANOL-FED RATS

被引：33

作者：

WORRALL, S

DEJERSEY, J

SHANLEY, BC

WILCE, PA

机构：

[1] Alcohol Research Unit, Department of Biochemistry, University of Queensland

来源：

ALCOHOL AND ALCOHOLISM | 1991年 / 26卷 / 04期

关键词：

D O I：

10.1093/oxfordjournals.alcalc.a045136

中图分类号：

R194 [卫生标准、卫生检查、医药管理];

学科分类号：

摘要：

Liver cytosolic fractions, obtained from rats pair-fed diets supplemented with either ethanol or an isocaloric amount of sucrose for periods from 3 weeks to 27 months, were tested for the presence of acetaldehyde-modified proteins by immunoblotting, using a partially purified antiserum raised in rabbits against proteins modified by acetaldehyde in vitro. The antiserum reacted with a large number of proteins in cytosolic fractions from ethanol-fed rats but did not react with any proteins in the same fraction from control animals. The duration of the ethanol-containing diet did not appear to influence the number of proteins modified or the total amount of modification within the time range tested. These results indicate that many proteins in rat liver cytosol are targets for modification by acetaldehyde in vivo.

引用

页码：437 / 444

页数：8

共 24 条

[1]

ABEL LE, 1984, ALCOHOLISM ELDERLY, P35

[2] FORMATION OF ACETALDEHYDE ADDUCTS WITH ETHANOL-INDUCIBLE P450IIE1 INVIVO [J].