ENERGY-TRANSFER KINETICS IN C-PHYCOCYANIN FROM CYANOBACTERIUM WESTIELLOPSIS PROLIFICA STUDIED BY PUMP-PROBE TECHNIQUES

被引：13

作者：

XIA, AD ^{[1
]}

ZHU, JC ^{[1
]}

JIANG, LJ ^{[1
]}

LI, DL ^{[1
]}

ZHANG, XY ^{[1
]}

机构：

[1] ACAD SINICA,CHANGCHUN INST PHYS,CHANGCHUN 130021,PEOPLES R CHINA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 179卷 / 01期

关键词：

D O I：

10.1016/0006-291X(91)91407-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The relaxation processes of C-phycocyanin at different aggregates have been investigated by pump-probe techniques. The lifetimes of ground state recovery measured at various wavelengths are analyzed by computer fitting of the kinetic data to a sum of three and four exponentials for monomers and trimers according to the nonlinear least-square principle, respectively. The shortest lifetime (about 56ps) is due to βs→βf transfer in one monomer, that decreases to 31ps in trimer due to the opening of new transfer channels. The second fastest component (about 151ps) in monomer is attributed tentatively to distribution of excitation energy between α and βf chromophores, that decreases to about 117ps in trimer caused by redistribution of excitation energy between them. The two long-lived components (about 690ps and 1385ps for monomer, 620ps and 1320ps for trimer) from some kinds of heterogeneity in some chromophores, such as α and βf chromophores which are emitting, show an equat amplitude ratio of 1:2 in both monomer and trimer. © 1991.

引用

页码：558 / 564

页数：7

共 12 条

[1] NUMBER AND DISTRIBUTION OF CHROMOPHORE TYPES IN NATIVE PHYCOBILIPROTEINS [J].

DALE, RE ;

TEALE, FWJ .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1970, 12 (02) :99-&

[2]

GANTT E, 1980, INT REV CYTOL, V66, P45

[3] PHYCOBILISOME - A MACROMOLECULAR COMPLEX OPTIMIZED FOR LIGHT ENERGY-TRANSFER [J].

GLAZER, AN .

BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 768 (01) :29-51

[4]

KOPAINSKY B, 1980, OPT COMMUN, V32, P45

[5] FUNCTIONAL ASSIGNMENT OF CHROMOPHORES AND ENERGY-TRANSFER IN C-PHYCOCYANIN ISOLATED FROM THE THERMOPHILIC CYANOBACTERIUM MASTIGOCLADUS-LAMINOSUS [J].

MIMURO, M ;

FUGLISTALLER, P ;

RUMBELI, R ;

ZUBER, H .

BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 848 (02) :155-166

[6] EXCITATION TRANSFER IN C-PHYCOCYANIN - FORSTER TRANSFER RATE AND EXCITON CALCULATIONS BASED ON NEW CRYSTAL-STRUCTURE DATA FOR C-PHYCOCYANINS FROM AGMENELLUM-QUADRUPLICATUM AND MASTIGOCLADUS-LAMINOSUS [J].

SAUER, K ;

SCHEER, H .

BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 936 (02) :157-170

[7] X-RAY CRYSTALLOGRAPHIC STRUCTURE OF THE LIGHT-HARVESTING BILIPROTEIN C-PHYCOCYANIN FROM THE THERMOPHILIC CYANOBACTERIUM MASTIGOCLADUS-LAMINOSUS AND ITS RESEMBLANCE TO GLOBIN STRUCTURES [J].

SCHIRMER, T ;

BODE, W ;

HUBER, R ;

SIDLER, W ;

ZUBER, H .

JOURNAL OF MOLECULAR BIOLOGY, 1985, 184 (02) :257-277

[8] CRYSTAL-STRUCTURE ANALYSIS AND REFINEMENT AT 2.5-A OF HEXAMERIC C-PHYCOCYANIN FROM THE CYANOBACTERIUM AGMENELLUM-QUADRUPLICATUM - THE MOLECULAR-MODEL AND ITS IMPLICATIONS FOR LIGHT-HARVESTING [J].

SCHIRMER, T ;

HUBER, R ;

SCHNEIDER, M ;

BODE, W ;

MILLER, M ;

HACKERT, ML .

JOURNAL OF MOLECULAR BIOLOGY, 1986, 188 (04) :651-676

[9] PURIFICATION AND PROPERTIES OF UNICELLULAR BLUE-GREEN ALGAE (ORDER CCHROOCOCCALES) [J].

STANIER, RY ;

KUNISAWA, R ;

MANDEL, M ;

COHENBAZ.G .

BACTERIOLOGICAL REVIEWS, 1971, 35 (02) :171-&

[10] ENERGY-TRANSFER AMONG THE CHROMOPHORES OF C-PHYCOCYANIN FROM ANABAENA-VARIABILIS USING STEADY-STATE AND TIME-RESOLVED FLUORESCENCE SPECTROSCOPY [J].

SWITALSKI, SC ;

SAUER, K .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1984, 40 (04) :423-427

← 1 2 →