COMBINATION OF INVITRO CAPPING AND RIBONUCLEASE PROTECTION IMPROVES THE DETECTION OF TRANSCRIPTION START SITES IN CHLOROPLASTS

被引：26

作者：

VERA, A ^{[1
]}

SUGIURA, M ^{[1
]}

机构：

[1] NAGOYA UNIV, CTR GENE RES, NAGOYA 46401, JAPAN

来源：

PLANT MOLECULAR BIOLOGY | 1992年 / 19卷 / 02期

关键词：

CHLOROPLAST RNA; INVITRO CAPPING; PROMOTER; RIBONUCLEASE PROTECTION;

D O I：

10.1007/BF00027352

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A primary transcript from the chloroplast rpl32 gene was labelled at its 5' end using a capping enzyme and [alpha-P-32]GTP followed by hybridization to a cold RNA probe. A RNase protection assay gave a protected band and its initiation site of transcription could thus be estimated, which had not been possible by using DNA probes. The combination of in vitro capping and RNase protection is an excellent method for mapping transcription initiation sites on the chloroplast genome and shows a high improvement relative to the DNA-employing strategies.

引用

页码：309 / 311

页数：3

共 13 条

[1]

BERK AJ, 1989, METHOD ENZYMOL, V180, P334

[2]

HALEY J, 1990, PLANT CELL, V2, P323, DOI 10.1105/tpc.2.4.323

[3]

HANLEYBOWDOIN L, 1985, MOL CELL BIOL, V5, P2733, DOI 10.1128/MCB.5.10.2733

[4] INITIATION AND PROCESSING OF ATP6, T-URF13 AND ORF221 TRANSCRIPTS FROM MITOCHONDRIA OF T-CYTOPLASM MAIZE [J].