ON THE FUNCTIONAL-ROLE OF THE TYR-639 RESIDUE OF BACTERIOPHAGE T7-RNA POLYMERASE

被引：11

作者：

RECHINSKY, VO ^{[1
]}

KOSTYUK, DA ^{[1
]}

TUNITSKAYA, VL ^{[1
]}

KOCHETKOV, SN ^{[1
]}

机构：

[1] VA ENGELHARDT MOLEC BIOL INST,VAVILOV ST 32,MOSCOW 117984,USSR

来源：

FEBS LETTERS | 1992年 / 306卷 / 2-3期

关键词：

T7-RNA POLYMERASE; MUTANT FORM OF ENZYME; NTP BINDING;

D O I：

10.1016/0014-5793(92)80983-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Substitution of Asp for a Tyr residue normally present at position 639 of the bacteriophage T7 RNA polymerase leads to a drastic drop in the enzymatic activity. This mutation does not affect the enzyme-promoter interaction but decreases the ability of the RNA polymerase to discriminate between GTP and ATP molecules, resulting in a decrease in the rate of the incorporation of the nucleotide into the RNA chain.

引用

页码：129 / 132

页数：4

共 16 条

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