CHARACTERIZATION OF THE HUMAN ROD TRANSDUCIN ALPHA-SUBUNIT GENE

被引：20

作者：

FONG, SL ^{[1
]}

机构：

[1] INDIANA UNIV,DEPT BIOCHIM & BIOL MOLEC,INDIANAPOLIS,IN 46202

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 11期

关键词：

D O I：

10.1093/nar/20.11.2865

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The human rod transducin alpha-subunit (T(r-alpha)) gene has been cloned. A cDNA clone, HG14, contained a 1.1 kb insertion when compared with the human T(r-alpha) cDNA published by Van Dop et al. (1). Based on two overlapping clones isolated from a human genomic library, the human T(r-alpha) gene is 4.9 kb in length and consists of nine exons interrupted by eight introns. Northern blots of human retina total RNA showed that the gene is transcribed by rod photoreceptors into two species of mRNA, 1.3 kb and 2.4 kb in size. Apparently, this is the result of alternative splicing. Two putative transcription initiation sites were determined by primer extension and Sl nuclease protection assays. The putative promoter regions of the human and mouse T(r-alpha) genes have an identity of 78.1%. As found in the mouse gene (2), no TATA consensus sequence is present in the human gene.

引用

页码：2865 / 2870

页数：6

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