DEVELOPMENT OF AN ANTIIDIOTYPIC ANTIBODY-REACTIVE WITH AN ANTIBODY DEFINING THE EPITOPE R P A P IN THE MUC-1 EPITHELIAL MUCIN CORE

被引:7
作者
BASHFORD, JL
ROBINS, RA
PRICE, MR
机构
[1] UNIV NOTTINGHAM HOSP,QUEENS MED CTR,DEPT IMMUNOL,NOTTINGHAM NG7 2UH,ENGLAND
[2] UNIV NOTTINGHAM,CANC RES LABS,NOTTINGHAM NG7 2RD,ENGLAND
[3] UNIV NOTTINGHAM,DEPT PHARMACEUT SCI,NOTTINGHAM NG7 2RD,ENGLAND
关键词
D O I
10.1002/ijc.2910540512
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
C595 is a murine IgG3 monoclonal antibody (MAb) raised against human urinary mucin. C595 antibody binds to the protein core of the MUC-1 mucin (the polymorphic epithelial mucin, PEM) which is elevated in tissue and secretions from human breast carcinomas. The antibody defines the tetrameric epitope, R P A P. In the present study, a syngeneic anti-idiotypic MAb, 911, was raised in BALB/c mice against the C595 antibody. This IgG2a anti-idiotypic antibody blocked the binding of C595 to its mucin core antigen. In turn, pre-blocking of C595 with a 20 amino-acid mucin core peptide specifically inhibited binding of 91 1 antibody to C595. Cross-reactivity of antibody 91 1 was only observed against the IgM MAb, 789/9 1, which has the same minimum antibody-binding epitope as C595, namely, R P A P. Syngeneic and xenogeneic anti-sera against 91 1 antibody displayed increased binding to heptameric peptide sequences containing the motif, R P A(P). The anti-idiotypic antibody 911 therefore appears to recognize a site within or close to the binding site of the C595 antibody and thus carries an internal image of the parental mucin epitope. Consequently, the 91 1 idiotypic network offers a promising model system to investigate the mechanism of anti-idiotype-induced tumour immunity. (C) 1993 Wiley-Liss, Inc.
引用
收藏
页码:778 / 783
页数:6
相关论文
共 21 条
[1]  
BRIGGS S, 1993, IN PRESS EUROP J CAN
[2]   A MONOCLONAL-ANTIBODY AGAINST HUMAN COLONIC ADENOMA RECOGNIZES DIFUCOSYLATED TYPE-2-BLOOD-GROUP CHAINS [J].
BROWN, A ;
FEIZI, T ;
GOOI, HC ;
EMBLETON, MJ ;
PICARD, JK ;
BALDWIN, RW .
BIOSCIENCE REPORTS, 1983, 3 (02) :163-170
[3]   MONOCLONAL-ANTIBODY THERAPY FOR THE INDUCTION OF TRANSPLANTATION TOLERANCE [J].
COBBOLD, SP .
IMMUNOLOGY LETTERS, 1991, 29 (1-2) :117-121
[4]   A MONOCLONAL-ANTIBODY, NCRC-11, RAISED TO HUMAN-BREAST CARCINOMA .1. PRODUCTION AND IMMUNOHISTOLOGICAL CHARACTERIZATION [J].
ELLIS, IO ;
ROBINS, RA ;
ELSTON, CW ;
BLAMEY, RW ;
FERRY, B ;
BALDWIN, RW .
HISTOPATHOLOGY, 1984, 8 (03) :501-516
[5]  
ERTL HCJ, 1988, VACCINE, V8, P80
[6]   IMMUNOPOTENTIATING EFFECTS OF THE ADJUVANTS SGP AND QUIL-A .1. ANTIBODY-RESPONSES TO T-DEPENDENT AND T-INDEPENDENT ANTIGENS [J].
FLEBBE, LM ;
BRALEYMULLEN, H .
CELLULAR IMMUNOLOGY, 1986, 99 (01) :119-127
[7]   ANTIBODIES TO MAJOR HISTOCOMPATIBILITY ANTIGENS PRODUCED BY HYBRID CELL LINES [J].
GALFRE, G ;
HOWE, SC ;
MILSTEIN, C ;
BUTCHER, GW ;
HOWARD, JC .
NATURE, 1977, 266 (5602) :550-552
[8]   STRUCTURE AND BIOLOGY OF A CARCINOMA-ASSOCIATED MUCIN, MUC1 [J].
GENDLER, SJ ;
SPICER, AP ;
LALANI, EN ;
DUHIG, T ;
PEAT, N ;
BURCHELL, J ;
PEMBERTON, L ;
BOSHELL, M ;
TAYLORPAPADIMITRIOU, J .
AMERICAN REVIEW OF RESPIRATORY DISEASE, 1991, 144 (03) :S42-S47
[9]   STRATEGIES FOR EPITOPE ANALYSIS USING PEPTIDE-SYNTHESIS [J].
GEYSEN, HM ;
RODDA, SJ ;
MASON, TJ ;
TRIBBICK, G ;
SCHOOFS, PG .
JOURNAL OF IMMUNOLOGICAL METHODS, 1987, 102 (02) :259-274
[10]  
HARLOW E, 1988, ANTIBODIES LABORATOR, P340