NITRIC-OXIDE SYNTHASE IN CEREBROVASCULAR ENDOTHELIAL-CELLS IS INHIBITED BY BREFELDIN-A

被引：7

作者：

STANBOLI, A

MORIN, AM

机构：

[1] VET ADM MED CTR,NEUROL RES LAB 111N,SEPULVEDA,CA 91343

[2] UNIV CALIF LOS ANGELES,SCH MED,DEPT NEUROL,LOS ANGELES,CA 90024

来源：

NEUROSCIENCE LETTERS | 1994年 / 171卷 / 1-2期

关键词：

NITRIC OXIDE SYNTHASE; CEREBROVASCULAR ENDOTHELIUM; BREFELDIN; GOLGI COMPLEX;

D O I：

10.1016/0304-3940(94)90641-6

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Nitric oxide synthase (NOS) is present within cerebrovascular endothelial cells in a distinct membrane-bound juxtanuclear location. The enzyme product, nitric oxide, causes vasodilation as well as stimulation of ADP-ribosylations of some proteins. The activity of specific stimulatory ADP-ribosylation factors, associated with the Golgi complex (GC), has been shown to be blocked by brefeldin A (BFA). We present evidence that BFA disperses the GC, disrupts NOS/NADPH diaphorase staining and inhibits NOS activity in addition to its previously described activities.

引用

收藏

页码：209 / 212

页数：4

相关论文

共 29 条

[1] ISOLATION OF NITRIC-OXIDE SYNTHETASE, A CALMODULIN-REQUIRING ENZYME [J].

BREDT, DS ;

SNYDER, SH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (02) :682-685

[2]

BRUNE B, 1989, J BIOL CHEM, V264, P8455

[3]

CARSON MP, 1986, IN VITRO CELL DEV B, V22, P344

[4] NITRIC-OXIDE STIMULATES THE ADP-RIBOSYLATION OF ACTIN IN HUMAN NEUTROPHILS [J].

CLANCY, RM ;

LESZCZYNSKAPIZIAK, J ;

ABRAMSON, SB .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 191 (03) :847-852

[5] BREFELDIN-A REDISTRIBUTES RESIDENT AND ITINERANT GOLGI PROTEINS TO THE ENDOPLASMIC-RETICULUM [J].

DOMS, RW ;

RUSS, G ;

YEWDELL, JW .

JOURNAL OF CELL BIOLOGY, 1989, 109 (01) :61-72

[6] MONO ADP-RIBOSYLATION OF TRANSDUCIN CATALYZED BY ROD OUTER SEGMENT EXTRACT [J].

EHRETHILBERER, S ;

NULLANS, G ;

AUNIS, D ;

VIRMAUX, N .

FEBS LETTERS, 1992, 309 (03) :394-398

[7] ENDOTHELIUM-DERIVED RELAXING FACTOR RELEASE ON ACTIVATION OF NMDA RECEPTORS SUGGESTS ROLE AS INTERCELLULAR MESSENGER IN THE BRAIN [J].

GARTHWAITE, J ;

CHARLES, SL ;

CHESSWILLIAMS, R .

NATURE, 1988, 336 (6197) :385-388

[8] 2 DISTINCT POPULATIONS OF ARF BOUND TO GOLGI MEMBRANES [J].

HELMS, JB ;

PALMER, DJ ;

ROTHMAN, JE .

JOURNAL OF CELL BIOLOGY, 1993, 121 (04) :751-760

[9] GOLGI DETECTION IN MITOTIC AND INTERPHASE CELLS BY ANTIBODIES TO SECRETED GALACTOSYLTRANSFERASE [J].

HILLER, G ;

WEBER, K .

EXPERIMENTAL CELL RESEARCH, 1982, 142 (01) :85-94

[10] NEURONAL NADPH DIAPHORASE IS A NITRIC-OXIDE SYNTHASE [J].

HOPE, BT ;

MICHAEL, GJ ;

KNIGGE, KM ;

VINCENT, SR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (07) :2811-2814