PREIMPLANTATION EMBRYO SEXING BY POLYMERASE CHAIN-REACTION AMPLIFICATION OF THE SRY GENE ON SINGLE-MOUSE BLASTOMERES

被引:8
作者
MULDER, LC
SACCO, MG
MANGIARINI, L
BROWN, J
COLLOTTA, A
VILLA, A
DEGIOVANNI, AM
VEZZONI, P
CLERICI, L
机构
[1] COMMISS EUROPEAN COMMUNITIES, ENVIRONM INST LIFE SCI, JOINT RES CTR, ISPRA, ITALY
[2] NATL COUNCIL RES, INST ADV BIOMED RES, MILAN, ITALY
[3] UNIV MILAN, INST GEN ANIM TECHNOL, MILAN, ITALY
来源
GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1993年 / 10卷 / 06期
关键词
D O I
10.1016/1050-3862(93)90010-G
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Accurate and rapid sex determination of preimplantation embryos has great potential both in animal breeding and in human pathology, rn the past, sex determination has been. accomplished by cytogenetic or immunologic means and by polymerase chain reaction amplification of Y-chromosome-specific repetitive sequences. More recently, amplification of the Y-specific single-copy ZFY gene has been used in humans for sex determination of preimplantation embryos. The experiments reported here indicate that another Y-chromosome-specific single-copy gene, the sex-determining region gene (sry) can be successfully amplified from single mouse blastomeres. Blastocysts positive for sry amplification were reimplanted to foster mothers, and sir of six newborns were male. We conclude that sry gene amplification can represent a good marker for embryo sex determination.
引用
收藏
页码:147 / 149
页数:3
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