STEREOSPECIFIC ANALYSIS OF MONOUNSATURATED TRIACYLGLYCEROLS IN COCOA BUTTER

被引:19
作者
TAKAGI, T [1 ]
ANDO, Y [1 ]
机构
[1] HOKKAIDO UNIV,FAC FISHERIES,DEPT CHEM,HAKODATE,HOKKAIDO 041,JAPAN
关键词
CHIRAL-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; COCOA BUTTER; PRIORITY FACTOR; STEREOSPECIFIC ANALYSIS; TRIACYLGLYCEROLS;
D O I
10.1007/BF02540989
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
The enantiomeric composition of the monounsaturated triacylglycerols (TG) from cocoa butter was estimated. The monounsaturated TG were separated into three fractions by reversed-phase high-performance liquid chromatography (HPLC), and each fraction was subjected to the stereospecific analysis with chiral-phase HPLC. The results indicated that the major TG consisted of equal amounts of 1-stearoyl-2-oleoyl-3-palmitoyl-sn-glycerol (SOP-sn-TG) and POS-sn-TG (47 mol%), 1,3-distearoyl-2-oleoyl-glycerol (SOS-TG) (33 mol%), and POP-TG (19 mol%). The contents of SOP-sn-TG and POS-sn-TG are 1.30 times that of the POP-TG content, and the SOS-TG content is 1.30(2) times that of the POP-TG content. The term ''priority factor'' is proposed for the ratio of the stearoyl group/palmitoyl group, 1.30 at the sn-l and sn-3 or 1(3)-position. It shows a distinct specificity for particular fatty acids or their Coenzyme A esters in random esterification at each position of the glycerol moiety in the biosynthesis of cocoa butter TG.
引用
收藏
页码:1203 / 1206
页数:4
相关论文
共 16 条
[1]   MICROMETHOD FOR STEREOSPECIFIC ANALYSIS OF TRIACYL-SN-GLYCEROLS BY CHIRAL-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
ANDO, Y ;
TAKAGI, T .
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1993, 70 (10) :1047-1049
[2]  
BROCKERH.H, 1967, J LIPID RES, V8, P167
[3]  
CHAISERI S, 1989, J AM OIL CHEM SOC, V66, P1772
[4]  
Christie W., 1986, ANAL OILS FATS, P313
[5]   SEPARATION OF MOLECULAR-SPECIES OF TRIACYLGLYCEROLS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH A SILVER ION COLUMN [J].
CHRISTIE, WW .
JOURNAL OF CHROMATOGRAPHY, 1988, 454 :273-284
[6]  
CHRISTIE WW, 1987, HIGH PERFORMANCE LIQ, pCH8
[7]  
Frentzen Margrit, 1993, P195
[8]  
GRIFFITHS G, 1991, PLANTA, V184, P279, DOI [10.1007/BF01102429, 10.1007/BF00197958]
[9]  
HENDRIKSE PW, 1994, LIPID HDB, P338
[10]   STEREOSPECIFIC ANALYSIS OF MAJOR TRIGLYCERIDE SPECIES IN MONOUNSATURATED FRACTION OF COCOA BUTTER [J].
SAMPUGNA, J ;
JENSEN, RG .
LIPIDS, 1969, 4 (06) :444-&