EFFECTS OF RUTHENIUM RED ON ACTIVATION OF CA-2+-DEPENDENT CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE

被引：16

作者：

MASUOKA, H

ITO, M

NAKANO, T

NAKA, M

TANAKA, T

机构：

[1] MIE UNIV,SCH MED,DEPT INTERNAL MED 1,TSU,MIE 514,JAPAN

[2] MIE UNIV,SCH MED,DEPT CHIRURG,TSU,MIE 514,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 169卷 / 01期

关键词：

D O I：

10.1016/0006-291X(90)91470-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ruthenium red inhibited Ca2+-dependent phosphodiesterase (Ca2+-PDE) selectively with an IC50 value of 15 μM. Increasing calmodulin concentration in the presence of both 100 μM and 4000 μM Ca2+ completely reversed the inhibition of Ca2+-PDE activity by ruthenium red. Ruthenium red-induced inhibition of Ca2+-PDE activity was also overcome by increasing the concentration of Ca2+ in the presence of both 200 ng and 2000 ng calmodulin, in sharp contrast to fluphenazine-induced inhibition of Ca2+-PDE. These results indicate that ruthenium red has distinct inhibitory mechanism which differs from that of calmodulin antagonists previously reported. © 1990.

引用

页码：315 / 322

页数：8

共 12 条

[1] RUTHENIUM RED SELECTIVELY PREVENTS CAPSAICIN-INDUCED NOCICEPTOR STIMULATION [J].