LARGE-SCALE PURIFICATION OF MEMBRANE-CONTAINING BACTERIOPHAGE PRD1 AND ITS SUBVIRAL PARTICLES

被引：52

作者：

BAMFORD, JKH ^{[1
]}

BAMFORD, DH ^{[1
]}

机构：

[1] UNIV HELSINKI, DEPT GENET, ARKADIANKATU 7, SF-00100 HELSINKI 10, FINLAND

来源：

VIROLOGY | 1991年 / 181卷 / 01期

基金：

芬兰科学院;

关键词：

D O I：

10.1016/0042-6822(91)90501-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

PRD1 is a dsDNA virus that infects Escherichia coli and Salmonella typhimurium. The genome is a linear molecule with 5′ covalently linked terminal protein. The virus has a lipid membrane inside the protein coat. We describe the large-scale purification of the virus using a zonal rotor and the yields and quality of the virus and its subviral assemblies for subsequent biophysical measurements. © 1991.

引用

页码：348 / 352

页数：5

共 24 条

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