PURIFICATION OF FORM L(2)-RUBISCO FROM A MARINE OBLIGATELY AUTOTROPHIC HYDROGEN-OXIDIZING BACTERIUM, HYDROGENOVIBRIO-MARINUS STRAIN MH-110

被引:20
作者
CHUNG, SY [1 ]
YAGUCHI, T [1 ]
NISHIHARA, H [1 ]
IGARASHI, Y [1 ]
KODAMA, T [1 ]
机构
[1] UNIV TOKYO, DEPT AGR CHEM, BUNKYO KU, TOKYO 113, JAPAN
关键词
RIBULOSE BIS-PHOSPHATE CARBOXYLASE; L(2)-RUBISCO; HYDROGENOVIBRIO-MARINUS;
D O I
10.1111/j.1574-6968.1993.tb06142.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) was purified from an obligately autotrophic hydrogen-oxidizing bacterium, Hydrogenovibrio marinus MH-110. The protein has a M(r) value of approximately 110000, and is composed of two identical subunits of 55 000. To our knowledge, the existence of L2-form RubisCO in a chemolithoautotrophic bacterium is first reported in this paper. The N-terminal amino acid sequence determination of the purified enzyme showed high homology with those of the L2-form RubisCO of Rhodospirillum rubrum and the L(x)-form RubisCO from Rhodobacter sphaeroides.
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页码:49 / 54
页数:6
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