SIMULTANEOUS DETERMINATION OF CREATININE AND URIC-ACID IN SERUM BY HIGH-PERFORMANCE ION-EXCHANGE CHROMATOGRAPHY WITH DIRECT INJECTION

被引：6

作者：

ETOH, T ^{[1
]}

IWATAKE, M ^{[1
]}

MIYAZAKI, M ^{[1
]}

HARADA, K ^{[1
]}

NAKAYAMA, M ^{[1
]}

SUGII, A ^{[1
]}

UJI, Y ^{[1
]}

OKABE, H ^{[1
]}

机构：

[1] KUMAMOTO UNIV,SCH MED,DEPT LAB MED,KUMAMOTO 860,JAPAN

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1992年 / 15卷 / 09期

关键词：

D O I：

10.1080/10826079208018309

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An anion-exchange chromatographic method for simultaneous determination of creatinine and uric acid in human serum is described. Serum samples were analyzed by direct injection high performance liquid chromatography which a column of quaternized resin cross-linked with ethylene glycol dimethacrylate (PCMS-Q). This allowed all proteins to elute at void volume. Thus, small analytes were readily separated from macromolecules. The method was found to be precise and accurate. There was a good correlation between the results of the new method and those of spectrophotometric methods for measuring creatinine and uric acid in human serum.

引用

页码：1565 / 1575

页数：11

共 12 条

[1]

BABB AL, 1972, T AM SOC ART INT ORG, V18, P98

[2] SEMIPERMEABLE-SURFACE REVERSED-PHASE MEDIA FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].