RAPD-BASED GENETIC-LINKAGE MAP OF BLUEBERRY DERIVED FROM A CROSS BETWEEN DIPLOID SPECIES (VACCINIUM-DARROWI AND VACCINIUM-ELLIOTTII)

被引:53
作者
ROWLAND, LJ
LEVI, A
机构
[1] Fruit Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, Beltsville, Maryland
关键词
MOLECULAR MARKERS; RANDOM AMPLIFIED; POLYMORPHIC DNA; POLYMERASE CHAIN REACTION;
D O I
10.1007/BF00221139
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
An initial genetic linkage map for blueberry has been constructed from over 70 random amplified polymorphic DNA (RAPD) markers that segregated 1:1 in a testcross population of 38 plants. The mapping population was derived from a cross between two diploid blueberry plants: the F, interspecific hybrid (Vaccinium darrowi Camp x V. elliottii Chapm.) and another V. darrowi plant. The map currently comprises 12 linkage groups (in agreement with the basic blueberry chromosome number) and covers a total genetic distance of over 950 cM, with a range of 3-30 cM between adjacent markers. The use of such a map for identifying molecular markers linked to genes controlling chilling requirement and cold hardiness is discussed.
引用
收藏
页码:863 / 868
页数:6
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