MOLECULAR-CLONING AND ADHESIVE PROPERTIES OF MURINE PLATELET ENDOTHELIAL-CELL ADHESION MOLECULE-1

被引：88

作者：

XIE, Y

MULLER, WA

机构：

[1] Lab. of Cell. Physiol. and Immunol., Rockefeller University, New York

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 12期

关键词：

IMMUNOGLOBULIN GENE SUPERFAMILY; CD31; LEUKOCYTES; MEMBRANE PROTEIN;

D O I：

10.1073/pnas.90.12.5569

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We describe the isolation and characterization of a functional murine platelet/endothelial cell adhesion molecule (PECAM) 1 cDNA done from a mouse lung library. At the nucleotide level, the coding sequence of murine PECAM-1 is 73% identical to human PECAM-1, and at the amino acid level, the sequence is 79% homologous to its human counterpart. Southern hybridization reveals that one copy of the gene exists in the mouse genome; Northern hybridization reveals a single mRNA species in mouse lung tissue. COS-7 and mouse L cells transfected with murine PECAM-1 expressed a 130-kDa glycoprotein on their surfaces that reacted with anti-murine PECAM-1 monoclonal antibody and comigrated on SDS/PAGE with human PECAM-1. Stable L-cell transfectants aggregate with each other in a PECAM-dependent, homophilic manner.

引用

页码：5569 / 5573

页数：5

共 28 条

[1] MOLECULAR AND CELLULAR PROPERTIES OF PECAM-1 (ENDOCAM/CD31) - A NOVEL VASCULAR CELL CELL-ADHESION MOLECULE [J].