GLUTAMATE TRANSPORTERS IN GLIAL PLASMA-MEMBRANES - HIGHLY DIFFERENTIATED LOCALIZATIONS REVEALED BY QUANTITATIVE ULTRASTRUCTURAL IMMUNOCYTOCHEMISTRY

被引：672

作者：

CHAUDHRY, FA ^{[1
]}

LEHRE, KP ^{[1
]}

CAMPAGNE, MV ^{[1
]}

OTTERSEN, OP ^{[1
]}

DANBOLT, NC ^{[1
]}

STORMMATHISEN, J ^{[1
]}

机构：

[1] UNIV OSLO, INST ANAT, N-0317 OSLO, NORWAY

来源：

NEURON | 1995年 / 15卷 / 03期

关键词：

D O I：

10.1016/0896-6273(95)90158-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The glutamate transporters GLT-1 and GLAST were studied by immunogold labeling on ultrathin sections of rat brain tissue embedded in acrylic resins at low temperature after freeze substitution, Both proteins were selective markers of astrocytic plasma membranes. GLT-1 was much higher in hippocampal astrocytes than in cerebellar astrocytes. Astroglial membrane GLAST densities ranked as follows: Bergmann > cerebellar granular layer approximate to hippocampus > cerebellar white matter. No astrocyte appeared unlabeled. Astrocytic membranes facing capillaries, pia, or stem dendrites were lower in glutamate transporters than those facing nerve terminals, axons, and spines. Parallel fiber boutons (glutamatergic) synapsing on interneuron dendritic shafts were surrounded by lower transporter densities than those synapsing on Purkinje cell spines. Our findings suggest the localizations of glutamate transporters are carefully regulated.

引用

页码：711 / 720

页数：10

共 27 条

[1] ARRIZA JL, 1994, J NEUROSCI, V14, P5559
[2] NEUROBIOLOGY - GLIA AND NEURONS IN DIALOG
ATTWELL, D
[J]. NATURE, 1994, 369 (6483) : 707 - 708
[3] NONVESICULAR RELEASE OF NEUROTRANSMITTER
ATTWELL, D
BARBOUR, B
SZATKOWSKI, M
[J]. NEURON, 1993, 11 (03) : 401 - 407
[4] PROLONGED PRESENCE OF GLUTAMATE DURING EXCITATORY SYNAPTIC TRANSMISSION TO CEREBELLAR PURKINJE-CELLS
BARBOUR, B
KELLER, BU
LLANO, I
MARTY, A
[J]. NEURON, 1994, 12 (06) : 1331 - 1343
[5] FREEZE-SUBSTITUTION AND LOWICRYL HM20 EMBEDDING OF FIXED RAT-BRAIN - SUITABILITY FOR IMMUNOGOLD ULTRASTRUCTURAL-LOCALIZATION OF NEURAL ANTIGENS
CAMPAGNE, MV
OESTREICHER, AB
VANDERKRIFT, TP
GISPEN, WH
VERKLEIJ, AJ
[J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1991, 39 (09) : 1267 - 1279
[6] CASADO M, 1993, J BIOL CHEM, V268, P27313
[7] AN [NA+ +K+]COUPLED L-GLUTAMATE TRANSPORTER PURIFIED FROM RAT-BRAIN IS LOCATED IN GLIAL-CELL PROCESSES
DANBOLT, NC
STORMMATHISEN, J
KANNER, BI
[J]. NEUROSCIENCE, 1992, 51 (02) : 295 - 310
[8] THE HIGH-AFFINITY UPTAKE SYSTEM FOR EXCITATORY AMINO-ACIDS IN THE BRAIN
DANBOLT, NC
[J]. PROGRESS IN NEUROBIOLOGY, 1994, 44 (04) : 377 - 396
[9] PURIFICATION AND RECONSTITUTION OF THE SODIUM-COUPLED AND POTASSIUM-COUPLED GLUTAMATE TRANSPORT GLYCOPROTEIN FROM RAT-BRAIN
DANBOLT, NC
PINES, G
KANNER, BI
[J]. BIOCHEMISTRY, 1990, 29 (28) : 6734 - 6740
[10] DEMONSTRATION OF GLUTAMATE/ASPARTATE UPTAKE ACTIVITY IN NERVE-ENDINGS BY USE OF ANTIBODIES RECOGNIZING EXOGENOUS D-ASPARTATE
GUNDERSEN, V
DANBOLT, NC
OTTERSEN, OP
STORMMATHISEN, J
[J]. NEUROSCIENCE, 1993, 57 (01) : 97 - 111

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