ACETYLCHOLINE - RELEASE FROM NEURAL TISSUE AND IDENTIFICATION BY PYROLYSIS-GAS CHROMATOGRAPHY

被引：14

作者：

SCHMIDT, DE

SZILAGYI, PI

ALKON, DL

GREEN, JP

机构：

[1] Department of Pharmacology, Mount Sinai School of Medicine, New York

来源：

SCIENCE | 1969年 / 165卷 / 3900期

关键词：

D O I：

10.1126/science.165.3900.1370

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Gas chromatographic analysis showed acetylcholine to be the only choline ester released from innervated longitudinal muscle of the guinea pig ileum by electrical stimulation. The amount of acetylcholine measured by gas chromatography agreed almost exactly with that measured by bioassay. Denervated longitudinal muscle produced no acetylcholine, and treatment of the muscle with tetrodotoxin markedly reduced acetylcholine output. The method permits the recovery and quantitation of amounts of acetylcholine as low as 5 nanograms in 5 milliliters of tissue perfusates.

引用

页码：1370 / &

共 9 条

[1]

FRIESEN AJD, 1965, J PHARMACOL EXP THER, V148, P312

[2] EFFECTS OF TETRODOTOXIN ON INNERVATED SMOOTH MUSCLE PREPARATIONS [J].