EFFECT OF OESTROGEN ON ACTIVITY AND BINDING OF MULTIPLE FORMS OF HEXOKINASE IN RAT UTERUS

The effect of oestrogen on the activity and binding of hexokinase types I and II in the rat uterus has been studied.It was found that 4 hr after administration of oestradiol there was no change in the total tissue content of hexokinase; however, there was a change in the distribution between the particulate and soluble fraction, with an 80% increase in the amount of hexokinase type I bound to the particles. There was no change in the binding of hexokinase type II. Significant increases in the total hexokinase of the uterus were found at 12 hr. At 96 hr hexokinase type I had risen 3-fold in the soluble fraction and 15-fold in the particulate fraction; hexokinase type II increased 10-fold in both fractions. © 1969.