PURIFICATION OF 7 PROTEIN-SYNTHESIS INITIATION-FACTORS FROM KREBS-II ASCITES-CELLS

被引：29

作者：

TRACHSEL, H ^{[1
]}

ERNI, B ^{[1
]}

SCHREIER, MH ^{[1
]}

BRAUN, L ^{[1
]}

STAEHELIN, T ^{[1
]}

机构：

[1] BASEL INST IMMUNOL,CH-4058 BASEL,SWITZERLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 561卷 / 02期

关键词：

(Ascites cell; Purification); Initiation factor; Protein synthesis;

D O I：

10.1016/0005-2787(79)90156-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Seven protein synthesis initiation factors were isolated from Krebs II ascites cells using the procedures developed for the purification of the corresponding factors from rabbit reticulocytes. The ascites factors display identical characteristics in ion exchange chromatography and sucrose density gradient sedimentation. Based on their profiles in SDS polyacrylamide gels, the ascites factors have polypeptide profiles and molecular weights identical to those of the reticulocyte factors. Most significantly, each ascites factor is competent in replacing its corresponding reticulocyte factor in a reconstituted in vitro protein synthesizing system which is dependent on all seven factors. © 1979.

引用

页码：484 / 490

页数：7

共 19 条

[1] PROCESSING OF ADENOVIRUS 2-INDUCED PROTEINS [J].