AN INDUCIBLE EXPRESSION VECTOR FOR BOTH FISSION AND BUDDING YEAST

被引：51

作者：

PICARD, D ^{[1
]}

SCHENA, M ^{[1
]}

YAMAMOTO, KR ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO,DEPT BIOCHEM & BIOPHYS,SAN FRANCISCO,CA 94143

来源：

GENE | 1990年 / 86卷 / 02期

基金：

美国国家卫生研究院; 美国国家科学基金会; 新加坡国家研究基金会;

关键词：

controlled promoter; glucocorticoid receptor; Recombinant DNA; Saccharomyces cerevisiae; Schizosaccharomyces pombe; shuttle plasmid; steroids;

D O I：

10.1016/0378-1119(90)90287-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have developed a vector system for inducible gene expression in both fission yeast (Schizosaccharomyces pombe) and budding yeast (Saccharomyces cerevisiae). The autonomously replicating expression vector contains multiple glucocorticoid response elements, rendering a linked promoter inducible 20-70-fold by glucocorticoid hormones in the presence of the mammalian glucocorticoid receptor. A polylinker with several unique cloning sites allows insertion of cDNAs of interest. Glucocorticoids are gratuitous signalling molecules in yeast, exerting little or no effect on the expression of genes other than those fused to the regulated promoter. © 1990.

引用

页码：257 / 261

页数：5

共 20 条

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