FLUORESCAMINE USE IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF AMINOCAPROIC ACID IN SERUM

A sensitive and specific high‐performance liquid chromatographic determination of aminocaproic acid in serum is described. ω‐Aminocaprylic acid is used as an internal standard. To 10 μl of serum, 10 μl of the internal standard solution and 50 μl of ethanol are added. After centrifugation, a portion of the supernate is evaporated. The residue is dissolved in 750 μl of 50 mM dibasic sodium phosphate, and then 250 μl of fluorescamine in acetonitrile (35 mg/100 ml) is added. The reaction mixture is chromatographed using a column of octadecylsilane bonded to silica and 44% acetonitrile in 0.5 mM phosphoric acid as the eluent. Quantitation is achieved by monitoring either the absorbance of the effluent at 405 nm or the fluorescence of the compounds with a fluorometer equipped with a flowcell. The method is reproducible, simple, and fast and has a precision of 4.4%. Copyright © 1979 Wiley‐Liss, Inc., A Wiley Company