IMMUNOHISTOCHEMICAL LOCALIZATION OF CARBONIC-ANHYDRASE ISOENZYME-VI, ISOENZYME-II, AND ISOENZYME-I IN HUMAN PAROTID AND SUBMANDIBULAR GLANDS

被引:134
作者
PARKKILA, S
KAUNISTO, K
RAJANIEMI, L
KUMPULAINEN, T
JOKINEN, K
RAJANIEMI, H
机构
[1] UNIV OULU, INST DENT, DEPT ANAT, KAJAANINTIE 52A, SF-90220 OULU, FINLAND
[2] UNIV OULU, DEPT OTOLARYNGOL, SF-90220 OULU, FINLAND
关键词
carbonic anhydrase; immunohistochemistry; saliva; salivary glands;
D O I
10.1177/38.7.2113069
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Human salivary carbonic anhydrase (HCA VI) was purified by inhibitor affinity chromatography and its location in the human parotid and submandibular glands identified, using a polyclonal antiserum raised against the purified enzyme in rabbits in conjunction with the peroxidase-antiperoxidase complex method. The antibodies raised against the purified enzyme in rabbits did not crossreact with the HCA II or I. However, they slightly recognized human IgA; the antiserum was therefore absorbed with human IgA before immunohistochemical use. HCA VI-specific staining was detected in the cytoplasm and particularly in the secretory granules of the serous acinar cells of both parotid and submandibular glands, the staining of the secretory granules being most distinct in paraformaldehyde-fixed tissues. Some epithelial cells and the luminal content of the striated ducts also gave a specific HCA VI staining. Staining specific for HCA II was also found in the granules of the serous acinar cells, particularly in the submandibular gland when Carnoy fluid fixation was used. Slight HCA II-specific staining was also detected in the striated ductal cells in the Carnoy fluid-fixed specimens. No staining specific for HCA I was detected. The results indicate that the serous acinar cells in human parotid and submandibular glands contain abundant HCA II and HCA VI. Interestingly, only HCA VI is secreted into the saliva, although both enzymes appear to be located in structures resembling the secretory granules in the acinar cells. The enzymes probably form a mutually complementary system regulating the salivary buffer capacity.
引用
收藏
页码:941 / 947
页数:7
相关论文
共 24 条
  • [1] BIOCHEMICAL DEMONSTRATION OF CARBONIC-ANHYDRASE IN THE RAT PAROTID SECRETION GRANULES
    CARPENTIER, P
    FOURNIE, J
    CHETAIL, M
    [J]. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1984, 429 (JUN) : 216 - 218
  • [2] CARPENTIER P, 1980, J BIOL BUCCALE, V8, P33
  • [3] CARBONIC-ANHYDRASE - ISOENZYMES, PROPERTIES, DISTRIBUTION, AND FUNCTIONAL SIGNIFICANCE
    CARTER, MJ
    [J]. BIOLOGICAL REVIEWS, 1972, 47 (04) : 465 - 513
  • [4] MITOCHONDRIAL CARBONIC-ANHYDRASE
    DODGSON, SJ
    FORSTER, RE
    STOREY, BT
    MELA, L
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (09): : 5562 - 5566
  • [5] FELDSTEIN JB, 1984, J BIOL CHEM, V259, P5447
  • [6] COMPLETE AMINO-ACID SEQUENCE OF OVINE SALIVARY CARBONIC-ANHYDRASE
    FERNLEY, RT
    WRIGHT, RD
    COGHLAN, JP
    [J]. BIOCHEMISTRY, 1988, 27 (08) : 2815 - 2820
  • [7] NOVEL CARBONIC-ANHYDRASE FROM THE OVINE PAROTID-GLAND
    FERNLEY, RT
    WRIGHT, RD
    COGHLAN, JP
    [J]. FEBS LETTERS, 1979, 105 (02): : 299 - 302
  • [8] IMMUNOLOCALIZATION OF CARBONIC-ANHYDRASE ISOZYMES IN RAT AND MOUSE SALIVARY AND EXORBITAL LACRIMAL GLANDS
    HENNIGAR, RA
    SCHULTE, BA
    SPICER, SS
    [J]. ANATOMICAL RECORD, 1983, 207 (04): : 605 - 614
  • [9] CARBONIC-ANHYDRASE IN MOUSE SALIVARY-GLANDS AND SALIVA - A HISTOCHEMICAL, IMMUNOHISTOCHEMICAL, AND ENZYME-ACTIVITY STUDY
    IKEJIMA, T
    ITO, S
    [J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1984, 32 (06) : 625 - 635
  • [10] KADOYA Y, 1987, Osaka City Medical Journal, V33, P99