CALCIUM-TRANSPORT BY PIGEON ERYTHROCYTE-MEMBRANE VESICLES

被引：9

作者：

TING, A ^{[1
]}

LEE, JW ^{[1
]}

VIDAVER, GA ^{[1
]}

机构：

[1] UNIV NEBRASKA,DEPT CHEM,LINCOLN,NE 68588

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 555卷 / 02期

基金：

美国国家卫生研究院;

关键词：

(Pigeon erythrocyte membrane vesicle); Ca[!sup]2+[!/sup] transport;

D O I：

10.1016/0005-2736(79)90164-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Membrane vesicles from pigeon erythrocytes show a rapid, ATP-dependent accumulation of 45Ca2+. Ca2+ accumulation ratios greater than or approximately equal to 104 are readily attained. For ATP-dependent Ca2+ uptake, V is 1.5 mmol · 1-1 · min-1 at 27°C (approx. 0.9 nmol · mg-1 protein · min-1), [Ca2+] 1 2 is 0.18 μM, [ATP] 1 2 is 30-60 μM, the Ca2+ uptake rate depends on [Ca2+]2 and the dependence of uptake rate on ATP concentration implies strong ATP-ATP cooperativity. The Arrhenius activation energy is 19.1 ± 1.4 kcal/mol and the pH optimum is approx. 6.9. © 1979.

引用

页码：239 / 248

页数：10

共 35 条

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[2] CHICKEN ERYTHROCYTE-MEMBRANES - COMPARISON OF NUCLEAR AND PLASMA-MEMBRANES FROM ADULTS AND EMBRYOS [J].