MITOGENIC ACTION OF INTERLEUKIN-1-ALPHA ON VASCULAR SMOOTH-MUSCLE CELLS MEDIATED BY PDGF

被引：78

作者：

IKEDA, U ^{[1
]}

IKEDA, M ^{[1
]}

OOHARA, T ^{[1
]}

KANO, S ^{[1
]}

YAGINUMA, T ^{[1
]}

机构：

[1] JICHI MED SCH,DEPT CLIN IMMUNOL,MINAMI KAWACHI,TOCHIGI 32904,JAPAN

来源：

ATHEROSCLEROSIS | 1990年 / 84卷 / 2-3期

关键词：

Ca [!sup]2+[!/sup; Interleukin-1; PDGF; Vascular smooth muscle cell;

D O I：

10.1016/0021-9150(90)90089-2

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We have investigated the effect of interleukin-1 (IL-1) on the growth of vascular smooth muscle cells (VSMC) isolated from rat aortae. Murine recombinant IL-la increased tritiated leucine incorporation into VSMC. IL-1 also stimulated tritiated thymidine uptake by VSMC in a dose-dependent manner. On the other hand, Ca2+-channel blocker, verapamil, inhibited the IL-1-induced thymidine uptake by VSMC with an IC50 of 10-8 M. Antibody specific for platelet-derived growth factor (PDGF) also totally inhibited the IL-1-induced thymidine uptake. IL-1 showed no effects on the intracellular CaZ+ level in VSMC. Above results support the premise that IL-1 promotes the growth of VSMC via induction of endogenous PDGF production and might thus participate in the abnormal proliferation of VSMC that occurs early in atherogenesis. © 1990.

引用

页码：183 / 188

页数：6

共 19 条

[1] ANGIOTENSIN-II-STIMULATED PROTEIN-SYNTHESIS IN CULTURED VASCULAR SMOOTH-MUSCLE CELLS [J].

BERK, BC ;

VEKSHTEIN, V ;

GORDON, HM ;

TSUDA, T .

HYPERTENSION, 1989, 13 (04) :305-314

[2] COEXPRESSION OF A PDGF-LIKE GROWTH-FACTOR AND PDGF RECEPTORS IN A HUMAN OSTEO-SARCOMA CELL-LINE - IMPLICATIONS FOR AUTOCRINE RECEPTOR ACTIVATION [J].

BETSHOLTZ, C ;

WESTERMARK, B ;

EK, B ;

HELDIN, CH .

CELL, 1984, 39 (03) :447-457

[3] CA-2+-CHANNEL BLOCKERS INHIBIT THE ACTION OF RECOMBINANT PLATELET-DERIVED GROWTH-FACTOR IN VASCULAR SMOOTH-MUSCLE CELLS [J].

BLOCK, LH ;

EMMONS, LR ;

VOGT, E ;

SACHINIDIS, A ;

VETTER, W ;

HOPPE, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (07) :2388-2392

[4] EXCHANGE CHARACTERISTICS OF THE NORADRENALIN-SENSITIVE CALCIUM STORE IN VASCULAR SMOOTH-MUSCLE CELLS OF RABBIT EAR ARTERY [J].

CASTEELS, R ;

DROOGMANS, G .

JOURNAL OF PHYSIOLOGY-LONDON, 1981, 317 (AUG) :263-279

[5] BIOLOGY OF INTERLEUKIN-1 [J].

DINARELLO, CA .

FASEB JOURNAL, 1988, 2 (02) :108-115

[6] STUDIES OF HYPERCHOLESTEROLEMIA IN THE NONHUMAN PRIMATE .1. CHANGES THAT LEAD TO FATTY STREAK FORMATION [J].

FAGGIOTTO, A ;

ROSS, R ;

HARKER, L .

ARTERIOSCLEROSIS, 1984, 4 (04) :323-340

[7] REPLICATION OF ARTERIAL SMOOTH-MUSCLE CELLS IN HYPERTENSION AND ATHEROSCLEROSIS [J].

GORDON, D ;

SCHWARTZ, SM .

AMERICAN JOURNAL OF CARDIOLOGY, 1987, 59 (02) :A44-A48

[8] IDENTIFICATION AND CHARACTERIZATION OF THE HIGH-AFFINITY VASCULAR ANGIOTENSIN-II RECEPTOR IN RAT MESENTERIC-ARTERY [J].

GUNTHER, S ;

GIMBRONE, MA ;

ALEXANDER, RW .

CIRCULATION RESEARCH, 1980, 47 (02) :278-286

[9] INHIBITION BY PROBUCOL OF INTERLEUKIN-1 SECRETION AND ITS IMPLICATION IN ATHEROSCLEROSIS [J].

KU, G ;

DOHERTY, NS ;

WOLOS, JA ;

JACKSON, RL ;

SCHMIDT, LF ;

HENDRICKS, D .

AMERICAN JOURNAL OF CARDIOLOGY, 1988, 62 (03) :B77-B81

[10] INTERLEUKIN-1 - A MITOGEN FOR HUMAN VASCULAR SMOOTH-MUSCLE CELLS THAT INDUCES THE RELEASE OF GROWTH-INHIBITORY PROSTANOIDS [J].

LIBBY, P ;

WARNER, SJC ;

FRIEDMAN, GB .

JOURNAL OF CLINICAL INVESTIGATION, 1988, 81 (02) :487-498

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