STEREOSPECIFIC HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF TOCAINIDE

被引：14

作者：

CARR, RA ^{[1
]}

FOSTER, RT ^{[1
]}

FREITAG, D ^{[1
]}

PASUTTO, FM ^{[1
]}

机构：

[1] UNIV ALBERTA,FAC PHARM & PHARMACEUT SCI,EDMONTON T6G 2N8,ALBERTA,CANADA

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 566卷 / 01期

关键词：

D O I：

10.1016/0378-4347(91)80120-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive high-performance liquid chromatographic assay was developed for the determination of tocainide enantiomers in plasma. Following extraction of tocainide from plasma, the enantiomers were derivatized with S-(+)-1-(1-naphthyl)ethylisocyanate. The resulting diastereomers were separated and quantified using normal-phase chromatography with fluorescence detection set at 220/345 nm (excitation/emission). The peaks, resolved with a resolution factor greater than 1.5, were free from interference. Linearity was established over the concentration range 0.25-10.0 mg/l for each enantiomer in plasma (r2 > 0.998). The inter-assay variability was less than 10% at all concentrations examined. The method can be used to determine the pharmacokinetics of tocainide enantiomers in man.

引用

页码：155 / 162

页数：8

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