ISOLATION AND STRUCTURAL CHARACTERIZATION OF NOVEL NEUTRAL OLIGOSACCHARIDE-ALDITOLS FROM RESPIRATORY-MUCUS GLYCOPROTEINS OF A PATIENT SUFFERING FROM BRONCHIECTASIS .1. STRUCTURE OF 11 OLIGOSACCHARIDES HAVING THE GLCNAC-BETA(1-]3)GAL-BETA(1-]4)GLCNAC-BETA(1-]6)GALNAC-OL STRUCTURAL ELEMENT IN COMMON

The carbohydrate chains of the respiratory-mucus glycoproteins of a patient (blood group O) suffering from bronchiectasis due to Kartagener's syndrome, were released by alkaline borohydride treatment of a pronase digest. Neutral oligosaccharides were obtained after ion-exchange chromatography and were subsequently separated utilizing gel filtration, HPLC on normal-phase alkylamine-bonded silica and reverse-phase HPLC, into 46 fractions. From these fractions oligosaccharide-alditols have been characterized by employing 500-MHz H-1-NMR spectroscopy, in conjunction with fast-atom-bombardment mass spectroscopy, methylation analysis and sugar analysis. Here 11 novel oligosaccharide structures are described. Five of them have the common element Fuc-alpha-(1 --> 2)Gal-beta-(1 --> 3)GlcNAc-beta-(1 --> 3)Gal-beta-(1 --> 4)GlcNAc-beta-(1 --> 6)GalNAc-ol, they are: [GRAPHICS] Another six possess Gal-beta-(1 --> 4)GlcNAc-beta-(1 --> 3)Gal-beta-(1 --> 4)GlcNAc-beta-(1 --> 6)GalNAc-ol as common element, they are: [GRAPHICS]