FUNCTION OF DIPEPTIDYL PEPTIDASE-IV (CD26, TP103) IN TRANSFECTED HUMAN T-CELLS

被引:33
作者
HEGEN, M
CAMERINI, D
FLEISCHER, B
机构
[1] UNIV MAINZ,DEPT MED 1,LANGENBECKSTR 1,W-6500 MAINZ,GERMANY
[2] MASSACHUSETTS GEN HOSP,DEPT MOLEC BIOL,BOSTON,MA 02114
关键词
D O I
10.1006/cimm.1993.1024
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
CD26 (Tp103) is a proteolytic enzyme (dipeptidyl peptidase IV) expressed on the T cell surface that defines an alternative activation signal for human T lymphocytes. It is absent from or present in only low amounts on resting T cells but it is expressed strongly after activation. Crosslinking of CD26/Tp103 via the monoclonal antibody CB.1 triggers functional activities in preactivated T cells. To study the molecular requirements for T cell activation via CD26 we transfected a cDNA encoding CD26 into several CD26-negative cells. In Jurkat T cell leukemia cells that normally do not express the CD26 antigen, the transfected CD26 molecule is functional because the monoclonal antibody CB.1 induces an increase of cytosolic Ca2+ concentration and IL-2 production. For this stimulatory effect a crosslinking of the monoclonal antibody CB.1 is necessary. After modulation of the TCR/CD3 complex the transfected Jurkat cells were insensitive to triggering via CD26. Moreover, a CD26-transfected TCR-negative variant of Jurkat cells did not respond to CD26 triggering despite high levels of expression of the molecule on their surface. These data demonstrate that the function of CD26/Tp103 is dependent on the expression of the T cell receptor complex. In search of a physiological function of CD26 we found a costimulatory effect of mAb CB.1 in combination with the nonstimulatory anti-CD3 antibody BMA030 and an additive effect in the response to the superantigen staphylococcal enterotoxin E. Transfected Jurkat cells, however, did not show a reproducibly enhanced responsiveness to the superantigen compared to that of untransfected cells. © 1993 Academic Press. All rights reserved.
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页码:249 / 260
页数:12
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