CULTURE OF BOVINE IVM IVF EMBRYOS UP TO BLASTOCYST STAGE IN DEFINED MEDIUM USING INSULIN, TRANSFERRIN AND SELENIUM OR GROWTH-FACTORS

被引：16

作者：

SHAMSUDDIN, M

LARSSON, B

RODRIGUEZMARTINEZ, H

机构：

[1] Department of Obstetrics and Gynaecology, Swedish University of Agricultural Sciences, Uppsala, S-750 07

来源：

REPRODUCTION IN DOMESTIC ANIMALS | 1993年 / 28卷 / 04期

关键词：

D O I：

10.1111/j.1439-0531.1993.tb00126.x

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The developmental competence of bovine IVM/IVF embryos was studied in a cell-free serum-tree culture system using a commercially available tissue culture medium (TCM 199) supplemented with BSA, insulin, transferrin and selenium. The data show that a semidefined medium can support the development of IVM/IVF embryos up to the blastocyst stage at a frequency and speed comparable to what can be seen in a complex Go-culture system.

引用

页码：209 / 210

页数：2