ENHANCED SURFACE-PLASMON RESONANCE INHIBITION TEST (ESPRIT) USING LATEX-PARTICLES

被引：52

作者：

SEVERS, AH

SCHASFOORT, RBM

机构：

[1] Department of Microbiology, TNO-Nutrition and Food Research, 3700 AJ Zeist

来源：

BIOSENSORS & BIOELECTRONICS | 1993年 / 8卷 / 7-8期

关键词：

SURFACE PLASMON RESONANCE; SUBMICRON LATEX PARTICLES; HUMAN CHORIONIC GONADOTROPIN; ESPRIT;

D O I：

10.1016/0956-5663(93)80075-Z

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The pregnancy hormone human chorionic gonadotropin (hCG) was used as a model antigen to describe a new assay, the Enhanced Surface Plasmon Resonance Inhibition Test (ESPRIT). It was shown that the introduction of sub-micron latex particles instead of anti-antibodies in an enhancement step improved the sensitivity of the assay by a factor of 30. Latex particles are therefore considered to be versatile tools in the development of new immunochemical assays for the detection of any analyte using SPR immunosensors.

引用

页码：365 / 370

页数：6

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