IMMUNOGOLD LOCALIZATION OF A DEVELOPMENTALLY-REGULATED, TAPETAL-SPECIFIC, 15 KDA LILY ANTHER PROTEIN

被引:12
作者
BALSAMO, RA [1 ]
WANG, JL [1 ]
ECKARD, KJ [1 ]
WANG, CS [1 ]
LORD, EM [1 ]
机构
[1] UNIV CALIF RIVERSIDE,DEPT BOT & PLANT SCI,RIVERSIDE,CA 92521
关键词
LILY; TAPETUM; ANTHER; PROTEIN; IMMUNOGOLD LOCALIZATION;
D O I
10.1007/BF01280288
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We have confirmed that the LLA-15 polypeptide of Lilium longiflorum is (a) tapetum specific with some expression possible in the adjacent middle layer cells and (b) relatively abundant as evidenced by the high density of gold particles localized to the tapetal cells. We have established that the protein is cytoplasmic and not associated with organelles, membranes, extracellular matrix or wall. We also report an amino acid composition of the molecule and a partial sequence which bears no resemblance to any protein yet described.
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页码:17 / 25
页数:9
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