ISOMERIZATION OF 5-ANDROSTEN-3,17-DIONE BY HEPATIC TISSUE

被引：6

作者：

KOERNER, DR

机构：

[1] Institute for Steroid Research, the Division of Neoplastic Medicine Montefiore Hospital, Medical Center, New York

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 176卷 / 02期

关键词：

D O I：

10.1016/0005-2760(69)90197-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The properties of the system in the soluble fraction of rat liver which catalyzes the conversion of 5-androsten-3,17-dione to the corresponding Δ4-3-ketone have been studied. Two components are required: a protein and a dialyzable organic molecule. These factors can be separated in good yield by filtration through a column of Bio-Gel P-2 and the protein component so obtained has been purified by ammonium sulphate fractionation and DEAE-cellulose chromatography. The dialyzable organic molecule eluted from the Bio-Gel P-2 column in the same position as NAD+ and NADH, but neither pyridine nucleotide could substitute for this component in the isomerization reaction. Isomerizing activity of a similar nature was found in the livers of the rabbit and guinea pig but no similar activity could be detected in beef, pig, sheep and chicken liver. © 1969.

引用

页码：383 / &

共 22 条

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