ALTERNATING LAMINATED ARRAY OF 2 TYPES OF MUCIN IN THE HUMAN GASTRIC SURFACE MUCOUS LAYER

被引:145
作者
OTA, H [1 ]
KATSUYAMA, T [1 ]
机构
[1] SHINSHU UNIV, SCH MED, DEPT LAB MED, ASAHI 3-1-1, MATSUMOTO, NAGANO 390, JAPAN
来源
HISTOCHEMICAL JOURNAL | 1992年 / 24卷 / 02期
关键词
D O I
10.1007/BF01082444
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Attempts have been made to develop a procedure for preserving and analysing the surface mucous layer of the human stomach in paraffin sections. Histologically normal gastric mucosae were obtained from 20 surgically removed stomachs. Of the different fixatives tested, Carnoy's solution gave rise to the most satisfactory results. In Haematoxylin-Eosin stained sections, the surface mucous layer appeared as a thick eosinophilic layer coating the gastric mucosal surface and measured 55.4 +/- 2.5-mu-m in the fundus and 21.8 +/- 1.0-mu-m in the pylorus respectively. A dual staining method consisting of galactose oxidase-cold thionine Schiff and paradoxical concanavalin A staining was applied to the surface mucous layer in order to reveal the distribution pattern of mucins secreted by two types of mucous cell in the gastric mucosa: surface mucous cells and gland mucous cells. As a result of this staining, an alternating laminated layer was visualized which consisted of the particular two types of mucin. In five cases, the surface mucous layer was examined in unfixed frozen sections. This layer was only partially preserved but revealed the same laminated structure. These results indicated that gland mucous cell mucins contribute to form the surface mucous layer.
引用
收藏
页码:86 / 92
页数:7
相关论文
共 22 条
[1]   MUCUS GLYCOPROTEIN STRUCTURE, GEL FORMATION AND GASTROINTESTINAL MUCUS FUNCTION [J].
ALLEN, A ;
HUTTON, DA ;
PEARSON, JP ;
SELLERS, LA .
CIBA FOUNDATION SYMPOSIA, 1984, 109 :137-156
[2]   THE GASTRIC-MUCOSAL EPITHELIAL BARRIER - ROLE OF MUCUS AND FIBRIN [J].
ALLEN, A ;
SELLERS, LA ;
BENNETT, MK .
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY, 1987, 22 :6-13
[3]   THE ROLE OF MUCUS IN THE PROTECTION OF THE GASTRODUODENAL MUCOSA [J].
ALLEN, A ;
HUTTON, DA ;
LEONARD, AJ ;
PEARSON, JP ;
SELLERS, LA .
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY, 1986, 21 :71-78
[4]   ADHERENT AND SOLUBLE MUCUS IN THE STOMACH AND DUODENUM [J].
ALLEN, A ;
CARROLL, NJH .
DIGESTIVE DISEASES AND SCIENCES, 1985, 30 (11) :S55-S62
[5]  
ALLEN A, 1989, HDB PHYSL GASTROINTE, V3, P359
[6]  
Babkin B. P., 1944, JAMA-J AM MED ASSOC, V126, P266, DOI [10.1001/jama.1944.02850390064034, DOI 10.1001/JAMA.1944.02850390064034]
[7]  
BELL AE, 1982, ADV EXP MED BIOL, V144, P97
[8]  
GLASS GBJ, 1953, GASTROENTEROLOGY, V23, P636
[9]   GLYCOCONJUGATE HISTOCHEMISTRY OF THE RAT FUNDIC GLAND USING GRIFFONIA-SIMPLICIFOLIA AGGLUTININ-II DURING THE DEVELOPMENT [J].
IHIDA, K ;
SUGANUMA, T ;
TSUYAMA, S ;
MURATA, F .
AMERICAN JOURNAL OF ANATOMY, 1988, 182 (03) :250-256
[10]  
IIDA F, 1976, Gastroenterologia Japonica, V11, P175