TUMOR-GROWTH AND ADHERENCE CHANGE THE EXPRESSION OF MACROPHAGE MAC-2

被引：9

作者：

ASKEW, D ^{[1
]}

BURGER, CJ ^{[1
]}

ELGERT, KD ^{[1
]}

机构：

[1] VIRGINIA POLYTECH INST & STATE UNIV,DEPT BIOL,MICROBIOL & IMMUNOL SECT,BLACKSBURG,VA 24061

来源：

CANCER LETTERS | 1993年 / 69卷 / 01期

关键词：

ANIMAL LECTINS; CELL-CYCLE KINETICS; GENE EXPRESSION;

D O I：

10.1016/0304-3835(93)90034-7

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The galactose-specific animal lectin, Mac-2, has been identified in macrophage (Mphi) membrane, cytoplasmic, and nuclear fractions. Flow cytometric analyses showed that there is a decrease in membrane Mac-2 during tumor growth. After 24-h adherence there was an increase in the number of normal host (NH) and tumor-bearing host (TBH) Mac-2+ Mphi. Immunoblot analyses of NH and TBH Mphi identified changes in the subcellular localization of Mac-2. The increase in nuclear Mac-2 during tumor growth, and after prolonged adherence of NH and TBH Mphi, correlates with an increase in Mphi entering the late G1 phases of the cell cycle. Northern blot analyses showed an increase in Mac-2 mRNA during tumor growth, and an increase in NH and TBH Mphi after 24-h adherence. Tumor growth is able to manipulate the immune system through Mphi by causing a down-regulation in membrane Mac-2 and an up-regulation in intracellular Mac-2. NH and TBH Mphi respond to adherence by expressing increased membrane and nuclear Mac-2, but TBH Mphi response is lower.

引用

页码：67 / 74

页数：8

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